| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In this study, I examined three subjects to improve the technique ‘acquirement of Epigenome editing plants using grafting’.
1. Efficiency of epigenome editing technique. Transgenic improved donor lines producing preferential 24 nt siRNAs (increased about 1.5 hold in comparison with the original donor line) were produced. I clarified that it’s necessary to carry out grafting effectively, ex. preparing thickness of the scion-stock, to act epigenome editing. 2. Generic application. In five potato cultivars, regeneration from roots was carried out successfully. 3. Extension of applied plants -application to apple-. Techniques which were regeneration from roots in apple and direct induction of a root sucker in cultivated plant roots without tissue cultures were established.
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