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Isolation of a causative gene that increases total dry matter production in tomato

Research Project

Project/Area Number 18K05582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Ohyama Akio  国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 上級研究員 (10355612)

Co-Investigator(Kenkyū-buntansha) 松尾 哲  国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, チーム長 (20414675)
諏訪部 圭太  三重大学, 生物資源学研究科, 准教授 (50451612)
加賀谷 安章  三重大学, 地域イノベーション推進機構, 准教授 (20335152)
宮武 宏治  国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 主任研究員 (70442754)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsトマト / 収量 / バイオマス / 総乾物生産量
Outline of Final Research Achievements

NILs with segregated QTL regions were cultivated in a highly environmentally controlled greenhouse of the National Agriculture and Food Research Organization (Tsukuba). Yield component analysis of NILs indicated that both total fruit weight and total dry matter production (TDM) were significantly higher in the NIL containing the high-yield QTL. These results suggest that the total fruit weight was improved by the increase of the TDM in the NIL with the high-yield QTL. In addition, the mechanism of increase in the TDM was physiologically elucidated by growth analysis. We found the causative candidate gene by RNA-seq analysis of NILs, and constructed genome editing vectors for complementation testing of the proof of the gene. Transformation of tomato and Arabidopsis thaliana using these vectors was performed, and the phenotypes of transformed Arabidopsis are currently being observed.

Academic Significance and Societal Importance of the Research Achievements

光合成による同化産物(バイオマス量)の増大に関わる遺伝的要因については、極めて知見に乏しい。イネ等主要穀類では一部例があるが、双子葉植物では知る限りでは例が無い。本研究により、トマトにより初めてその遺伝子座が見出され、遺伝子座の効果が統計的に証明され、その効果が栽培生理学的に解明された点が第一の学術的意義となる。さらには、その候補遺伝子が同定された点も挙げられる。この遺伝子座の導入により、トマトの収量改善はもとより、さらには候補遺伝子が明らかになる事により、他のナス科植物並びに双子葉植物の収量を改変しうる可能性が提示できた点が第一の社会的意義となる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2018

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] QTL mapping of fruit nutritional and flavor components in tomato (Solanum lycopersicum) using genome-wide SSR markers and recombinant inbred lines (RILs) from an intra-specific cross2018

    • Author(s)
      Kimbara Junji、Ohyama Akio、Chikano Hiroshi、Ito Hirotaka、Hosoi Katsutoshi、Negoro Satomi、Miyatake Koji、Yamaguchi Hirotaka、Nunome Tsukasa、Fukuoka Hiroyuki、Hayashi Takeshi
    • Journal Title

      Euphytica

      Volume: 214 Issue: 11 Pages: 210-210

    • DOI

      10.1007/s10681-018-2295-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 高品質多収トマト育成における育種技術的展望2018

    • Author(s)
      大山 暁男、松永 啓、林 武司
    • Journal Title

      農業および園芸

      Volume: 93 Pages: 626-631

    • NAID

      40021613985

    • Related Report
      2018 Research-status Report
  • [Presentation] 多収性トマト品種‘鈴玉’を用いた至適栽培管理による多収生産の実証2018

    • Author(s)
      河崎 靖、松永 啓、牛島弘貴、斎藤 岳士、大山 暁男、東出 忠桐
    • Organizer
      園芸学会秋季大会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] ナス科植物における可食部への同化産物分配量の調節に関与する遺伝子及びその利用2021

    • Inventor(s)
      大山暁男、林武司、松尾哲、宮武宏治
    • Industrial Property Rights Holder
      大山暁男、林武司、松尾哲、宮武宏治
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-054293
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report
    • Overseas

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Published: 2018-04-23   Modified: 2024-12-25  

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