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Relationships among wintering ability, fructan content, and allelic variation of fructan metabolic enzyme genes in Japanese barley cultivars

Research Project

Project/Area Number 18K05604
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Nakata Masaru  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 上級研究員 (60649862)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsオオムギ / フルクタン / 越冬性 / DNAマーカー / 遺伝子型 / 耐雪性 / 遺伝子多型
Outline of Final Research Achievements

The relationships among wintering ability, shoot fructan content, and the allelic variation of five fructan metabolic enzyme genes in 105 Japanese barley cultivars and breeding lines were analyzed. 6 to 10 genotypes were found in each of the five fructan metabolic enzyme genes in the tested barley cultivars and lines. A significant correlation was detected between fructan content and wintering ability, specifically, cultivars and lines with a higher fructan content prior to snow cover exhibited a superior wintering ability. The cultivars and lines with the highest wintering ability possessed a unique 6-SFT genotype. The cultivars and lines were classified into three groups with a high, medium, and low wintering ability based on the 1-FEH genotypes. We developed dCAPS markers that identify the 6-SFT and 1-FEH genotypes observed in the group with the highest wintering ability and in the groups with a high, medium, and low wintering ability, respectively.

Academic Significance and Societal Importance of the Research Achievements

積雪の多い我が国の寒冷地で栽培されるオオムギ品種は、長期積雪下でも生存できる高度な耐雪性を備えなければならない。しかし、近年は少雪傾向にあるため、品種育成過程における耐雪性の優劣による選抜効率が低下しており、また、温暖地品種等から有用形質を取り入れる過程で本来寒冷地品種が有していた高度耐雪性を失う可能性が高い。
本研究で開発したDNAマーカーを用いることにより、高度耐雪性を有する系統を効率的に選抜でき、極端な多雪年でも甚大な被害を受けにくいオオムギ品種の効率的な開発が可能になる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019 2018 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] 国内オオムギ品種・系統の越冬性とフルクタン含量およびフルクタン代謝酵素遺伝子多型の関係2021

    • Author(s)
      中田克、関昌子、青木秀之、長嶺敬
    • Journal Title

      育種学研究

      Volume: 印刷中

    • NAID

      130008054177

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel allele of the ADP-glucose transporter gene in ‘Tanikei QM-1’ markedly increases health-promoting compounds in barley grains2018

    • Author(s)
      中田 克, 池田 達哉, 一ノ瀬 靖則, 野方 洋一, 関 昌子, 青木 秀之, 加藤 常夫, 小前 幸三, 長嶺 敬
    • Journal Title

      Breeding Research

      Volume: 20 Issue: 2 Pages: 124-132

    • DOI

      10.1270/jsbbr.18J06

    • NAID

      130007525274

    • ISSN
      1344-7629, 1348-1290
    • Year and Date
      2018-12-01
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 複数の機能性成分を増加させるlys5変異のオオムギ育種への利用2020

    • Author(s)
      中田克、関昌子、青木秀之、長嶺敬、池田達哉、野方洋一、一ノ瀬靖則
    • Organizer
      第15回 ムギ類研究会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] オオムギのフルクタン代謝が耐雪性に与える影響2020

    • Author(s)
      中田克,関昌子,青木秀之,長嶺敬
    • Organizer
      第61回 日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] オオムギのフルクタン関連酵素遺伝子群の品種間多型と耐雪性の関係2019

    • Author(s)
      中田克, 関昌子, 青木秀之, 長嶺敬
    • Organizer
      日本育種学会 第135回講演会
    • Related Report
      2018 Research-status Report
  • [Remarks] 農研機構成果情報「大麦の複数の機能性成分を増加させるADP-グルコーストランスポーター遺伝子」

    • URL

      http://www.naro.affrc.go.jp/project/results/4th_laboratory/carc/2018/carc18_s07.html

    • Related Report
      2020 Annual Research Report 2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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