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Analysis of molecular mechanism for self-compatibility in Chrysanthemum seticuspe

Research Project

Project/Area Number 18K05619
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionHiroshima University

Principal Investigator

Nakano Michiharu  広島大学, 統合生命科学研究科(理), 特任助教 (40705159)

Co-Investigator(Kenkyū-buntansha) 草場 信  広島大学, 統合生命科学研究科(理), 教授 (20370653)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsキク / 野生種 / 自家和合性 / ポジショナルクローニング / 自然変異 / キクタニギク / RNA-seq解析 / リシークエンス解析 / マッピング / 野生ギク / ゲノム解析
Outline of Final Research Achievements

In this study, we aimed to clarify the molecular mechanism related to Csc1, a self-compatibility locus found in a strain AEV02 of Chrysanthemum seticuspe. Using the BC1F1 population derived from crosses a cross between self-incompatible(SI) strain and AEV02-derived self-compatible(SC) strain, the candidate region for the Csc1 locus was narrowed to 850 kb. This region contains 18 genes, including two homologs of SRK, which encodes the S locus in Arabidopsis. One of those SRK homologs, SLPK_R expressed in the style of SI strains, but not expressed in the style of SC strains. Furthermore, SLPK_R had a possible nonsense mutation specifically for SC strains in the coding region. These results indicated SLPK_R is an important candidate for the Csc1 locus.

Academic Significance and Societal Importance of the Research Achievements

キク属は自家不和合性であり、このことがキク育種を困難としている一因である。以前の研究で自家和合性変異体を単離し、その変異を利用することで純系化したモデル系統Gojo-0の育成が行われてきたが、変異の実体は不明であった。本研究によりキク属で初めての自家和合性変異の実体解明に近づくことができたが、今後、この変異に関わる詳細な機構を明らかにすることができれば、キク育種に自家和合性を導入することで新たな育種システムの開発につながると期待される。またキク科では自家不和合性の詳細なメカニズムは明らかにされておらず、本研究の結果を元にキク科の自家不和合性機構の解明につながることが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2019

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] A pure line derived from a self-compatible Chrysanthemum seticuspe mutant as a model strain in the genus Chrysanthemum2019

    • Author(s)
      Nakano Michiharu、Taniguchi Kenji、Masuda Yu、Kozuka Toshiaki、Aruga Yuki、Han Jin、Motohara Koichiro、Nakata Masashi、Sumitomo Katsuhiko、Hisamatsu Tamotsu、Nakano Yoshihiro、Yagi Masafumi、Hirakawa Hideki、Isobe Sachiko N、Shirasawa Kenta、Nagashima Yumi、Na Haiyan、Chen Li、Liang Guolu、Chen Ruiyan、Kusaba Makoto
    • Journal Title

      Plant Science

      Volume: 287 Pages: 110174-110174

    • DOI

      10.1016/j.plantsci.2019.110174

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] NBRP広義キク属: キク属モデル系統の開発と植物多様性研究への展開2019

    • Author(s)
      中野道治・増田優・谷口研至・草場信
    • Journal Title

      BSJ-Review

      Volume: 10 Issue: C Pages: 148-157

    • DOI

      10.24480/bsj-review.10c6.00166

    • ISSN
      2432-9819
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo whole-genome assembly in Chrysanthemum seticuspe, a model species of Chrysanthemums, and its application to genetic and gene discovery analysis2019

    • Author(s)
      Hirakawa Hideki、Sumitomo Katsuhiko、Hisamatsu Tamotsu、Nagano Soichiro、Shirasawa Kenta、Higuchi Yohei、Kusaba Makoto、Koshioka Masaji、Nakano Yoshihiro、Yagi Masafumi、Yamaguchi Hiroyasu、Taniguchi Kenji、Nakano Michiharu、Isobe Sachiko N
    • Journal Title

      DNA Research

      Volume: In press Issue: 3 Pages: 1-9

    • DOI

      10.1093/dnares/dsy048

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A pure line derived from the self-compatible Chrysanthemum seticuspe mutant as a model strain in the genus Chrysanthemum2019

    • Author(s)
      Michiharu Nakano, Kenji Taniguchi, Yu Masuda, Yuki Aruga, Toshiaki Kozuka, Makoto Kusaba
    • Organizer
      26th International EUCARPIA Symposium Section Ornamentals: EDITING NOVELTY
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] キクタニギクゲノム情報を活用したChrysanthemum self-compatible1遺伝子座のマッピング2019

    • Author(s)
      中野道治・谷口研至・増田優・住友克彦・八木雅史・中野善公・久松完・磯部祥子・草場信
    • Organizer
      園芸学会 令和元年度秋季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] キクタニギクにおける自家和合性の遺伝解析2019

    • Author(s)
      中野道治・谷口研至・住友克彦・八木雅史・中野善公・久松完・磯部祥子・草場信
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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