Identification genomic regions which are responsible for pathogenicity of Verticillium dahliae

• Project/Area Number: 18K05641
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Chiba University
• Principal Investigator: Usami Toshiyuki 千葉大学, 大学院園芸学研究科, 准教授 (50334173)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 植物病原糸状菌 / 病原性 / 植物病害 / 土壌伝染病 / 菌類 / 植物病原菌

Outline of Final Research Achievements

Verticillium dahliae causes vascular wilt disease on various dicot plant species. A genomic region which is responsible for pathogenicity on tomato had been estimated in our previous study. In this study, we successfully estimated a genomic region which is responsible for pathogenicity on sweet pepper. Both genomic regions were analyzed and candidates for pathogenicity-determining genes were estimated. Host range of V. dahliae is probably determined by combination of these genomic sequences.

Academic Significance and Societal Importance of the Research Achievements

半身萎凋病菌は様々な種類の植物に感染する植物病原菌であるが、病原性を決定するメカニズムは解明されていない。本研究では、病原性の異なる菌株同士を遺伝的に交雑することで、本菌のトマトおよびピーマンに対する病原性を決定するゲノム領域を推定できた。また、その領域に存在する病原性決定遺伝子の候補を明らかにした。これを手がかりとして本菌の病原性メカニズムを解明できれば、病害を防ぐ方法の開発につながる。

Research Products

• [Journal Article] Identification of the chromosome region responsible for pathogenicity of Verticillium dahliae on tomato using genetic recombination through protoplast fusion (2020)
  • Author(s): Usami Toshiyuki、Iida Naoko、Nakao Keisuke、Hamano Ayame、Suzuki Nana、Ohmura Yusuke、Komiya Yoshimi
  • Journal Title: Journal of General Plant Pathology Volume: 86 Issue: 6 Pages: 477-485
  • DOI: 10.1007/s10327-020-00955-x
  • Peer Reviewed
• [Presentation] Genomic region responsible for pathogenicity of Verticillium wilt pathogen on bell pepper (2020)
  • Author(s): Chen, Z. , Usami, T.
  • Organizer: III Asian Horticultural Congress
  • Int'l Joint Research
• [Presentation] 半身萎凋病原菌のピーマンに対する病原性を決定するゲノム領域の推定 (2020)
  • Author(s): 陳崢・佐藤優貴子・宇佐見俊行
  • Organizer: 日本土壌微生物学会2020年度大会
• [Presentation] トマト半身萎凋病菌の病原性決定染色体の推定と解析４ (2020)
  • Author(s): 添田悠美・山本沙季・宇佐見俊行
  • Organizer: 令和2年度 日本植物病理学会大会