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Proteomic study of plant epidermal tissues in response to phytopathogen invasion

Research Project

Project/Area Number 18K05642
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionKanazawa University

Principal Investigator

Nishiuchi Takumi  金沢大学, 学際科学実験センター, 准教授 (20334790)

Co-Investigator(Kenkyū-buntansha) 玉置 大介  富山大学, 学術研究部理学系, 助教 (20793053)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物免疫 / 植物表皮 / プロテオミクス / 病原性糸状菌 / 表皮 / 病原糸状菌 / 侵入抵抗性 / 気孔 / 赤かび病菌
Outline of Final Research Achievements

The plant epidermis is the first line of plant defence against pathogen invasion and likely contains important regulatory proteins related to the plant-pathogen interaction. We performed comparative proteomic studies to identify differentially expressed proteins in the leaf epidermis challenged with fungal phytopathogen. The conidia solutions were dropped onto the Arabidopsis leaf surface and then collect the epidermal tissues from inoculated and mock treated leaves at 4 and 24 hpi. Label free quantification methods showed that proteins related to defence signaling, transcription factors, and cell wall organization were up-regulated in the inoculated leaf epidermis. More than 300 fungal proteins expressed on the Arabidopsis epidermis were also identified and about 13% of them were predicted as effector-like proteins. These results suggested that our method is useful for understanding the early expressions of plant and fungal proteins related to their interactions.

Academic Significance and Societal Importance of the Research Achievements

植物と病原菌の相互作用にはタンパク質が重要な役割を担っており、感染過程で発現変動を示すタンパク質を明らかにすることが、植物を病気から守る上で重要な基盤的知見となります。本研究では、病原菌を接種した植物の表皮組織のみを用いて網羅的なタンパク質の発現解析を行う実験系を構築し、感染初期に発現応答を示す多くのタンパク質を同定することに成功しました。今後は、同定したタンパク質の機能を解明することで、植物と病原菌の相互作用における分子機構を紐解いていきます。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (15 results)

All 2021 2020 2019 2018 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Nicotinamide Effectively Suppresses Fusarium Head Blight in Wheat Plants2021

    • Author(s)
      Sidiq Yasir、Nakano Masataka、Mori Yumi、Yaeno Takashi、Kimura Makoto、Nishiuchi Takumi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 6 Pages: 2968-2968

    • DOI

      10.3390/ijms22062968

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection.2020

    • Author(s)
      Asano T, Nguyen HT, Yasuda M, Sidiq Y, Nishimura K, Nakashita H, Nishiuchi T.
    • Journal Title

      J Exp Bot.

      Volume: 71 Issue: 6 Pages: 2085-2097

    • DOI

      10.1093/jxb/erz556

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Secretome Study at Plant-Pathogen Interface2021

    • Author(s)
      Yasir Sidiq, Takumi Nishiuchi
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 安全で効果的な赤かび病防除に向けた植物由来の新規抵抗性誘導剤の開発2019

    • Author(s)
      西内 巧
    • Organizer
      日本マイコトキシン学会第 81回学術講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Proteomic study of plant leaf epidermis challenged with Fusarium graminearum.2019

    • Author(s)
      Yasir Sidiq, Daisuke Tamaoki, Takumi Nishiuchi
    • Organizer
      IS-MPMI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Proteomic analysis of leaf epidermis inoculated with Fusarium graminearum in Arabidopsis ein3 mutant.2019

    • Author(s)
      Daisuke Tamaoki, Daishi Iekada, Yasir Sidiq, Ichiro Karaha, Takumi Nishiuchi
    • Organizer
      IS-MPMI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Ethylene signaling negatively regulates the stomata movement by infection of Fusarium graminearum2019

    • Author(s)
      Daishi Iekada, Takumi Nishiuchi, Ichiro Karaha, Daisuke Tamaoki
    • Organizer
      IS-MPMI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Heat-shock treatment induces SA-dependent NPR1-independent disease resistance in Arabidopsis2019

    • Author(s)
      Rina Horita, Mai Inoue, Tomoka Fujita, Miyuki Kusajima, Takumi Nishiuchi, Hideo Nakashita
    • Organizer
      IS-MPMI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Type II phosphatidate phosphatase gene CoPAP2 of Colletotrichum orbiculare is involved in fungal pathogenesis and host defense responses.2019

    • Author(s)
      Sayo Kodama, Takumi Nishiuchi, Yasuyuki Kubo
    • Organizer
      IS-MPMI
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ムギ類赤かび病菌に抵抗性を示すシロイヌナズナein3-1変異体における表皮プロテオーム 解析2019

    • Author(s)
      玉置大介、池田大志、唐原一郎、西内巧
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シロイヌナズナの表皮におけるムギ類赤かび病菌接種によるタンパク質の発現変動の解析2018

    • Author(s)
      玉置大介,池田大志,唐原一郎,西内巧
    • Organizer
      日本植物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ムギ類赤かび病菌を接種したシロイヌナズナの葉の表皮におけるプロテオーム2018

    • Author(s)
      玉置大介、西内巧
    • Organizer
      北陸線植物バイオサイエンス研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] エチレン非感受性変異体 ein3-1 におけるムギ類赤かび病菌に対する病害抵抗性の定量解析2018

    • Author(s)
      池田大志、西内巧、玉置大介
    • Organizer
      北陸線植物バイオサイエンス研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Proteome analysis of plant epidermis challenged with phytopathogen.2018

    • Author(s)
      Takumi Nishiuchi
    • Organizer
      International Joint Conference on Genetics and Medicine
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 金沢大学遺伝子研究施設西内研究室

    • URL

      https://nishiuchitakumi.com/

    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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