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Development of disease-resistant rice plants using epigenetic mutations and verification of their practical use

Research Project

Project/Area Number 18K05644
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionMie University

Principal Investigator

Kobayashi Issei  三重大学, 地域イノベーション推進機構, 教授 (90205451)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords病害抵抗性 / イネ / エピジェネティクス / DNAメチル化
Outline of Final Research Achievements

We have developed a method for orienting epigenetic variation in rice by regenerated plants from callus treated with a plant activator. In this study, we conducted experiments to prove that the resistance conferred by this method is due to epigenetic mutation and to demonstrate its genetic stability and practicality. Analysis of the methylation status of Nipponbare and each line revealed that the resistant lines acquired specific DNA methylation changes. By comparing these results with RNA-seq results, we identified three genes whose expression changes correlated with DNA methylation changes in promoter regions. Based on these results, we have applied for an international patent for the method of plant production using this method.

Academic Significance and Societal Importance of the Research Achievements

世界の人口増加と新興国における畜産物の需要拡大は食料事情の逼迫を招き、2050年までに食料生産を倍増させる必要があるとされている。一方、地球環境の急激な変化は、生物的・非生物的ストレスを作物に与え、食料生産の重大な脅威となっている。したがって、将来の食料確保にはストレス耐性植物の開発が急務であると言える。しかし、現行の交配育種はゲノムDNAにランダムに生じる突然変異に依存するため、長い開発期間と莫大なコストを要する。我々の発見は、エピジェネティック変異を利用して目的とするストレス耐性形質に標的を狙って改善することが可能な世界初の技術に発展する可能性が高い。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish2020

    • Author(s)
      Okamoto Ryuji、Goto Itaru、Nishimura Yuhei、Kobayashi Issei、Hashizume Ryotaro、Yoshida Yoshinori、Ito Rie、Kobayashi Yuhko、Nishikawa Misato、Ali Yusuf、Saito Shunsuke、Tanaka Toshio、Sawa Yoshiki、Ito Masaaki、Dohi Kaoru
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 10 Pages: 0240129-0240129

    • DOI

      10.1371/journal.pone.0240129

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物へのエピジェネティック変異の人為的方向付けに関与する分子機構2019

    • Author(s)
      池田 昂志、小林 裕子、小林 一成
    • Organizer
      地域イノベーション学会第9回学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] エピジェネティック変異の人為的誘導によるいもち病抵抗性イネの作出2018

    • Author(s)
      亀田裕作、小林裕子、小林一成
    • Organizer
      地域イノベーション学会第8回学術大会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 遺伝子の発現が誘導された非天然の植物およびその生産方法2020

    • Inventor(s)
      小林一成
    • Industrial Property Rights Holder
      小林一成
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 遺伝子の発現が誘導された非天然の植物を生産する方法2019

    • Inventor(s)
      小林一成
    • Industrial Property Rights Holder
      小林一成
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-083683
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-12-25  

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