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Molecular mechanisms on plant viral synergism mediated by salicylic acid glucosyltransferase

Research Project

Project/Area Number 18K05655
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionUniversity of Miyazaki

Principal Investigator

Takeshita Minoru  宮崎大学, 農学部, 教授 (00304767)

Co-Investigator(Kenkyū-buntansha) 増田 税  北海道大学, 農学研究院, 教授 (60281854)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsplant virus / synergy / SAGT / cucumber mosaic virus / turnip mosaic virus / Plant virus / Synergy / シナジー / CMV / TuMV / SA / 2b / サリチル酸配糖化酵素遺伝子 / サリチル酸 / サリチル酸配糖化酵素 / キュウリモザイクウイルス / カブモザイクウイルス
Outline of Final Research Achievements

Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in ASM (BTH)-treated Nicotiana benthamiana plants than in SA-treated N. benthamiana and water-treated control plants. ASM treatment also significantly suppressed systemic symptoms after infection with cucumber mosaic virus (CMV). The results indicated that SAGT has an important role in the SA-mediated host defense responses. In addition, SAGT transcript levels under co-infection with CMV and turnip mosaic virus (TuMV) in N. benthamiana showed significant difference in comparison to those in single infection with CMV, TuMV, and non-inoculated healthy control. These results together indicate that SAGT has a role in plant viral synergism between CMV and TuMV.

Academic Significance and Societal Importance of the Research Achievements

本研究では植物ウイルス間の病原性相乗効果(シナジー)について追究した。シナジーは植物ウイルスが同じ個体に重複感染することで病徴の劇症化をもたらす減少であり、農作物のウイルス感染による被害を助長している。しかしながら、植物ウイルス間のシナジー現象はその詳細が明らかになっていないため、今回その分子機構の解明を目的とした。植物ウイルスの抵抗性誘導には植物ホルモンの1種であるサリチル酸(SA)の蓄積が重要である。そこで、本研究ではSAを非活性状態にするSAGT遺伝子の機能について注目した。SAGTの発現状況を解析した結果、SAGTの発現変動がシナジー現象に関与することが示唆された。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2021 2020 2019

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results)

  • [Journal Article] The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat protein2020

    • Author(s)
      Khaing, Y.Y., Kobayashi, Y., Takeshita, M.
    • Journal Title

      Virology Journal

      Volume: 17 Issue: 1 Pages: 49-49

    • DOI

      10.1186/s12985-020-01303-3

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The C-terminal region of the 2a protein and 2b protein of cucumber mosaic virus are involved in the induction of shoestring-like leaf blade in tomato2020

    • Author(s)
      Yu Yu Khaing et al.
    • Journal Title

      Virus Research

      Volume: 289 Pages: 198172-198172

    • DOI

      10.1016/j.virusres.2020.198172

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness.2020

    • Author(s)
      Kobayashi, Y., Fukuzawa, N., Hyodo, A., Kim, H., Mashiyama, S., Ogihara, T., Yoshioka, H., Matsuura, H., Masuta, C., Matsumura, T. and Takeshita, M.
    • Journal Title

      Molecular Plant Pathology

      Volume: 21 Issue: 3 Pages: 429-442

    • DOI

      10.1111/mpp.12906

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アシベンゾラル S メチルによるキュウリモザイクウイルス感染抑制効果の可視化2021

    • Author(s)
      小林優大、平野智也、増田 税、竹下 稔
    • Organizer
      令和3年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The 2b protein and the C-terminal region of the 2a protein of cucumber mosaic virus interfere with the microRNA pathway in tomato2020

    • Author(s)
      Yu Yu Khaing, Kobayashi, Y., Takeshita, M.
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The 2b protein and the C-terminal region of the 2a protein of cucumber mosaic virus interfere with the microRNA pathway in tomato2020

    • Author(s)
      Yu Yu Khaing, Yudai Kobayashi, Minoru Takeshita
    • Organizer
      日本植物病理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 小林優大・増山翔太・荻原 毅・松浦英幸・増田 税・竹下 稔2019

    • Author(s)
      BTHは植物防御応答で重要なサリチル酸配糖化酵素遺伝子の転写を抑制する
    • Organizer
      日本植物病理学会植物感染生理談話会
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物ウイルス間の病原性相乗作用に関する分子機構の解析2019

    • Author(s)
      大霜和倫・小田裕太・小林優大・夏秋知英・増田 税・竹下 稔
    • Organizer
      日本植物病理学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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