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Mode of action of fungistatic fungicide ferimzone: search for target pathways that circumvent the emergence of tolerant strains

Research Project

Project/Area Number 18K05658
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionRyukoku University

Principal Investigator

Okuno Tetsuro  龍谷大学, 農学部, 教授 (00221151)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords殺菌剤 / フェリムゾンゾン / 作用機作 / 銅トランスポーター / いもち病菌 / ウリ類炭疽病菌 / 静菌作用 / 耐性菌問題 / フェリムゾン / 薬剤耐性 / ウリ類炭疽病 / メラニン化 / イネいもち病菌 / 静菌的防除剤
Outline of Final Research Achievements

Circumventing the emergence of fungicide-resistant strains is a crucial issue for robust disease management in agriculture. The fungicide ferimzone has been used for the control of rice diseases including rice blast. The emergence of ferimzone-resistant strains in rice fields has not been reported. In this research, we identified the copper transport ICT1 gene and the CCC2 gene as the ferimzone sensitivity genes in Colletotrichum orbiculare and the rice blast fungus Magnaporthe oryzae. Genetic and cytological analyses showed that functional defects in the copper transport pathways, consisting of Ict1 and Ccc2, led to the low sensitivity to ferimzone and the pathogenicity defect due to attenuated melanization in the appressorium. Importantly, the presence of CuSO4 induced high sensitivity to ferimzone even in the ict1 mutant. This study shows that there is a trade-off relation between the sensitivity to ferimzone and fungal pathogenicity.

Academic Significance and Societal Importance of the Research Achievements

植物病害防除において殺菌剤の果たす役割は大きい。しかし、薬剤耐性菌株が多発して農業上大きな問題となっている。本研究では、イネ病害病防除剤として市販されてから約30年間、圃場で薬剤耐性菌株出現の報告のない静菌性殺菌剤フェリムゾンの作用機作研究を行い、薬剤感受性に関わる代謝系(銅輸送系)とその関連遺伝子を明らかにした。このことは、糸状菌病害防除において薬剤耐性菌の出現を回避できる薬剤ターゲット分子と代謝系を提示したことであり、その成果は新規薬剤開発にとって意義がある。また、フェリムゾン感受性と銅輸送系の関連性の発見は糸状菌におけるは銅輸送系ネットワーク解明の重要な情報源となると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2019

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results)

  • [Journal Article] Trade-Off Relation between Fungicide Sensitivity and Melanin Biosynthesis in Plant Pathogenic Fungi2020

    • Author(s)
      Ken Harata, Hiroyuki Daimon, and Tetsuro Okuno
    • Journal Title

      iScience

      Volume: 23 Issue: 11 Pages: 1-15

    • DOI

      10.1016/j.isci.2020.101660

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Threonine synthase CoTHR4 is involved in infection-related morphogenesis during the pre-penetration stage in Colletotrichum orbiculare2019

    • Author(s)
      Ken Harata, Tetsuro Okuno
    • Journal Title

      Microbial Pathogenesis

      Volume: 137 Pages: 1-9

    • DOI

      10.1016/j.micpath.2019.103746

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hijacking a host scaffold protein, RACK1, for replication of a plant RNA virus.2019

    • Author(s)
      Kiwamu Hyodo, Nobuhiro Suzuki and Tetsuro Okuno
    • Journal Title

      New Phytologist

      Volume: 221(2) Issue: 2 Pages: 935-945

    • DOI

      10.1111/nph.15412

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ウリ類炭疽病菌の病原性関連遺伝子GRIM-19の機能解析2020

    • Author(s)
      篠永隼・西山雄大・原田賢・奥野哲郎
    • Organizer
      日本植物病理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ウリ類炭疽病菌におけるフェリムゾン感受性とメラニン生合成の関係2019

    • Author(s)
      原田 賢, 奥野哲郎
    • Organizer
      日本農薬学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ウリ類炭疽病菌における銅輸送経路は殺菌剤フェリムゾン感受性に関与する2019

    • Author(s)
      原田 賢, 奥野哲郎
    • Organizer
      日本植物病理学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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