| Project/Area Number |
18K05664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Nishizawa Yoko 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, ユニット長 (40355756)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | いもち病菌 / イネ / 病原性 / イネ-いもち病菌相互作用 / 病原力遺伝子 / RBF1 / いもち病 / 感染機構 / 病原力因子 / エフェクター |
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| Outline of Final Research Achievements |
To elucidate the mechanism of the action the Rbf1 protein, which is specifically secreted from the rice blast fungus at the penetration stage, we analyzed the site of its action. We prepared the transgenic rice plants that expectedly accumulated Rbf1 in the intercellular spaces or the cytoplasm, and we evaluated their blast disease resistance. As a result, the rice plants introduced with the RBF1 gene with the secretion signal sequence showed significantly reduced resistance. The data suggested that Rbf1 functions at the intercellular spaces between rice and the blast fungus.
Next, we analyzed the expression of 10 chitin-elicitor-responsive genes using the transformed cell lines. The expression patterns induced after chitin-elicitor treatment were similar to that of the control line regardless of the presence or absence of the secretion signal sequence in the introduced RBF1 gene. Thus, it was unlikely that Rbf1 directly acts on the expression of rice disease responsive genes.
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| Academic Significance and Societal Importance of the Research Achievements |
いもち病はイネだけでなく、近年はコムギでの被害が拡大するなど、依然として防除すべき重要病害であるため、半活物寄生菌であるいもち病菌の感染機構を分子レベルで理解することは学術的のみならず経済的にも重要である。いもち病菌の病原力に大きな影響を及ぼす感染時特異的発現タンパク質Rbf1がイネ細胞内ではなく、イネ組織に侵入したいもち病菌糸の周辺で機能することを示唆した本研究成果は、今後の防除法の開発研究に資すると考えられる。
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