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Identification of bacterial wilt resistanse genes in solanaceous plants

Research Project

Project/Area Number 18K05666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research Institution岡山県農林水産総合センター生物科学研究所

Principal Investigator

Mukaihara Takafumi  岡山県農林水産総合センター生物科学研究所, その他部局等, 専門研究員 (80344406)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords青枯病 / 抵抗性 / エフェクター / NBS-LRR抵抗性タンパク質 / ナス / 抵抗性遺伝子 / Avr / トルバム・ビガー
Outline of Final Research Achievements

Although bacterial wilt is an important disease of solanaceous crops, there is little knowledge about resistance genes. We searched for eggplant lines collected from worldwide for bacterial wilt resistance, and found the eggplant line No. 98 with strong bacterial wilt resistance. The No. 98 line recognized an Avr effector from phylotype I Ralstonia solanacearum and exhibited strong resistance. We also succeeded in identifying an Avr effector from phylotype IV Ralstonia solanacearum that is recognized by eggplant and induces hypersensitive responses. Crossing experiments strongly suggested that the two bacterial wilt resistance genes found in this study were both dominant and considered to be NBS-LRR resistant protein genes.

Academic Significance and Societal Importance of the Research Achievements

青枯病はナス科作物の重要病害であるにも関わらず、抵抗性遺伝子に関する知見がほとんど無い。本研究ではナス青枯病菌(phylotype I)及びジャガイモ青枯病菌(phylotype IV)を材料に、これら菌株を認識する(抵抗性遺伝子を持つ)ナス系統と認識しない(抵抗性遺伝子を持たない)ナス系統をそれぞれ見出し、抵抗性系統が認識するAvrエフェクターの同定にも成功した。現在作成中の交配後代集団を利用して抵抗性遺伝子をクローニングできる可能性が極めて高い。また、No. 98系統が持つ抵抗性遺伝子は既存ナス品種に交配で容易に導入できると考えられ、育種利用価値が非常に大きいと考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] Ralstonia solanacearum Type III Effector RipAC Targets SGT1 to Suppress Effector-Triggered Immunity2020

    • Author(s)
      Nakano Masahito、Ichinose Yuki、Mukaihara Takafumi
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 12 Pages: 2067-2076

    • DOI

      10.1093/pcp/pcaa122

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HopH1 effectors of Pseudomonas syringae pv. tomato DC3000 and pv. syringae B728a induce HR cell death in nonhost eggplant Solanum torvum2020

    • Author(s)
      Nahar Kamrun、Mukaihara Takafumi、Taguchi Fumiko、Matsui Hidenori、Yamamoto Mikihiro、Toyoda Kazuhiro、Noutoshi Yoshiteru、Shiraishi Tomonori、Ichinose Yuki
    • Journal Title

      Journal of General Plant Pathology

      Volume: 87 Issue: 1 Pages: 24-29

    • DOI

      10.1007/s10327-020-00961-z

    • NAID

      120007163672

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The type III effector RipB from Ralstonia solanacearum RS1000 acts as a major avirulence factor in Nicotiana benthamiana and other Nicotiana species.2019

    • Author(s)
      Nakano, M. and Mukaihara, T.
    • Journal Title

      Molecular Plant Pathology

      Volume: 20 Issue: 9 Pages: 1237-1251

    • DOI

      10.1111/mpp.12824

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive identification of PTI suppressors in type III effector repertoire reveals that Ralstonia solanacearum activates jasmonate signaling at two different steps.2019

    • Author(s)
      Nakano, M. and Mukaihara, T.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 23 Pages: 5992-5992

    • DOI

      10.3390/ijms20235992

    • NAID

      120006816634

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ralstonia solanacearum type III effector RipAL targets chloroplasts and induces jasmonic acid production to suppress salicylic acid-mediated defense responses in plants2018

    • Author(s)
      Nakano, M. and Mukaihara, T.
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2576-2589

    • DOI

      10.1093/pcp/pcy177

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Role of trehalose synthesis in Ralstonia syzigii PW1001 to induce hypersensitive response on eggplant (Solanum melongena cv. Senryo-nigou)2021

    • Author(s)
      Laili, N., Mukaihara, T., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., and Ichinose, Y.
    • Organizer
      令和3年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ナス科植物に認識される新規な青枯病菌エフェクターの同定2019

    • Author(s)
      中野真人、向原隆文
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Presentation] ゲノム情報を活用したナス科作物の青枯病抵抗性遺伝子の探索2019

    • Author(s)
      向原隆文
    • Organizer
      岡山県立研究機関協議会第11回研究交流発表会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 植物ホルモン情報伝達経路を標的とする青枯病菌エフェクターの探索と機能解析2018

    • Author(s)
      中野真人、向原隆文
    • Organizer
      平成30年度日本植物病理学会関西部会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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