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New approach to non-self component recognition: elucidation of immune response regulation mechanisms based on target size.

Research Project

Project/Area Number 18K05669
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39050:Insect science-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Furukawa Seiichi  筑波大学, 生命環境系, 准教授 (10391583)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords細胞性免疫 / 貪食 / 包囲化 / アワヨトウ / トランスクリプトーム / 昆虫免疫 / 包囲化反応 / 遺伝子発現 / EPL / 転写調節領域 / 昆虫 / 血球細胞 / サイズ認識
Outline of Final Research Achievements

In response to pathogenic microorganisms and parasitic insects invading the insect hemocoel, hemocytes activate cellular immune reactions such as phagocytosis and encapsulation to deal with them. This study aimed to identify new cellular immune regulatory factors through transcriptome analysis. It also sought to elucidate the interactions between these newly identified factors and known regulatory factors in order to reveal the overall picture of the cellular immune system.
As a result, several novel gene groups that specifically control phagocytosis and encapsulation were obtained. Currently, the gene function analysis of these regulatory genes is being carried out, with the almost established RNAi method being used.

Academic Significance and Societal Importance of the Research Achievements

昆虫が地球上に繁栄しうる理由の一つとして、劣悪な環境でも生き延びることを可能とする強力な免疫系の存在が挙げられる。本研究により、体腔内に侵入してきた病原微生物や寄生性昆虫に対して活性化する細胞性免疫制御因子を複数同定することに成功した。これらの知見は、害虫防除において、生物農薬の効果を向上させることに寄与するとともに、病気に強い益虫の作出にも貢献できるだろう。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2019

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Cytotoxic effects of β‐asarone on Sf9 insect cells2019

    • Author(s)
      Thitaree Yooboon Kazumu Kuramitsu Vasakorn Bullangpoti Yooichi Kainoh Seiichi Furukawa
    • Journal Title

      Archives of insect biochemistry and physiology

      Volume: 102 Issue: 1

    • DOI

      10.1002/arch.21596

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection of Wolbachia Endosymbiont in Several Agriculturally Important Insect Parasitoids in Bogor, Indonesia2019

    • Author(s)
      Mahardika Gama Pradana, Giyanto, Seiichi Furukawa, Satoshi Nakamura and Damayanti Buchori
    • Journal Title

      AGRIVITA

      Volume: 41 Issue: 2 Pages: 364-371

    • DOI

      10.17503/agrivita.v41i2.1855

    • NAID

      120007133167

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ヤドリバエ幼虫のホスト脂肪体誘引物質分泌器官を突き止める2020

    • Author(s)
      張 凱・一木 良子・中村 達・古川 誠一
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] The Effect of Drino inconspicuoides Larvae on Suppressing the Immune System of the Hosts2020

    • Author(s)
      Schwier N, Kai Z, Nakamura S, Furukawa S
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] The microtype tachinid, Pales pavida (Meigen): escaping from host's immune responses resulting in deferent parasitization rates between host sexes2019

    • Author(s)
      Nakamura S, Furukawa S, Tanaka T
    • Organizer
      The 6th International Entomophagous Insects Conference(国際学会)
    • Related Report
      2019 Research-status Report
  • [Presentation] アワヨトウの包囲化促進レクチン遺伝子のプロモーター解析と発現局在解析2019

    • Author(s)
      松元 咲樹,古川 誠一.
    • Organizer
      第63回日本応用動物昆虫学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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