Production and evaluation research of universal eggs corresponding to the conservation of genetic resources by somatic cell reprogramming technology
| Project/Area Number |
18K05693
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39060:Conservation of biological resources-related
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| Research Institution | Kindai University |
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Principal Investigator |
Anzai Masayuki 近畿大学, 先端技術総合研究所, 教授 (30454630)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝資源保全 / 体細胞核移植 / マウス / 実験動物 / 動物園動物 / 野生動物 / 異種間 / 遺伝資源保存 / 展示動物 |
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| Outline of Final Research Achievements |
Wild animals may be used for research in the fields of medicine and basic biology. However, there is concern that genetic resources will be depleted due to the shrinking habitat. In this study, we constructed a universal ovum production and evaluation study corresponding to the conservation of genetic resources, it used the aged mouse and Apodemus speciosus-derived fibroblasts to fusion enucleated oocytes. Reconstructed oocytes were investigated for long exposure of donor cells in the egg cytoplasm and pretreatment with VC of the donor cells to further support the hypomethylated state, and 3 hours of exposure in the egg cytoplasm was the embryo. We have discovered that it may increase the developmental potential of some of the. It was suggested that these results can be applied to epigenetic genetic resource conservation research through somatic cell nuclear transfer technology.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、小分子の培地への添加によりマウス核移植胚の発生率を劇的に向上させる方法を開発した。この新規核移植法を用いて、従来法では初期化が困難であった野生マウス細胞の初期化を誘導する種々の条件検討を行なった結果、トリコスタチンA（TSA）およびビタミンC（VC）と脱イオンウシ血清アルブミン（d-BSA）で処理した胚の免疫細胞化学的染色により、H3K9トリメチル化の減少を示し細胞分裂の再開を誘起することが可能であった。これらの手法は、現存する動物組織からの遺伝的評価が可能であることを示すと共に新たな遺伝資源保存法を提示すると共に、最終的に、絶滅動物のゲノムを保存・増幅する手法開発が期待できる。
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Signs of biological activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging2019
Author(s)
Kazuo Yamagata, Kouhei Nagai, Hiroshi Miyamoto, ...., Tetsuya Handa (17番目), Hiroshi Kimura, Yoshihiko Hosoi, Tasuku Mitani, Kazuya Matsumoto, Akira Iritani
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Journal Title
Scientific Reports
Volume: 9 巻
Issue: 1
Pages: 4050-4050
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes2018
Author(s)
Morita K, Tokoro M, Hatanaka Y, Higuchi C, Ikegami H, Nagai K, Anzai M, Kato H, Mitani T, Taguchi Y, Yamagata K, Hosoi Y, Miyamoto K, Matsumoto K
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Journal Title
Journal of Reproduction and Development
Volume: 64
Issue: 2
Pages: 161-171
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed / Open Access
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[Presentation] キングペンギン(Aptenodytes patagonicus)由来精液を用いたグリセ ロール不含有凍結保存法の評価～凍結融解後の精子形態の一考～2019
Author(s)
安齋政幸, 野田義博, 石渡俊行, 大澤郁朗, 東 里香, 山鹿優真, 山﨑脩杜, 佐藤翔太, 斎藤勝彦, 尾崎美樹, 安達那央子
Organizer
第25回日本野生動物医学会大会
Related Report
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