| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Vitrification-based method was successfully established for storage at -196oC of Oomycetes. The optimal droplet vitrification method using Oomycetes for the storage at -196oC is as follows. Oomycete isolates were cultured with the rape seeds for 30 days at 25°C on solidified PDA medium. Rape seeds containing oomycete were placed in 15 ml petri dish and osmoprotected with LS (liquid medium supplemented with 1.0 M sucrose and 2.0 M glycerol) for 30 min. Then they were dehydrated PVS2 for 20 min at 25ºC. After dehydration placed in wells on an aluminum cryo-plate, and directly plunged into liquid nitrogen and maintained at -196 ºC. As results, three Oomycetes isolates were successfully cryopreserved with 100% viability. Vitrification-based protocol was cryopreservation method for reliable long-term preservation of Oomycetes resources.
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