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Study of carbon supply and utilization in xylem by isotopic imaging

Research Project

Project/Area Number 18K05758
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Takeuchi Miyuki  東京大学, 大学院工学系研究科(工学部), 主任研究員 (20378912)

Co-Investigator(Kenkyū-buntansha) 則定 真利子  東京大学, アジア生物資源環境研究センター(現在使用不可), 准教授 (00463886)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords木部形成 / 細胞壁 / 二次イオン質量分析法 / 同位体イメージング
Outline of Final Research Achievements

The distribution of carbon in trees after photosynthetic carbon assimilation was analyzed using stable isotopic labeling method. The isotopic tracer content of leaves, phloem and xylem was measured. Carbon transport in trees, and carbon distribution in different fractions such as cell wall and soluble organic carbon were analyzed. These results provided information on the carbon supply to xylem and the periodicity of cell wall formation in the tree. The detailed distribution of the isotopic tracer was observed by elemental imaging using a secondary ion mass spectrometer, and we analyzed the tracer distribution in combination with information on cell wall components.

Academic Significance and Societal Importance of the Research Achievements

炭素の供給は樹木の木質形成に大きな影響を与える大きな要因のひとつであるが、これまで炭素の供給と細胞壁形成を数時間単位や細胞レベルで調べた例はほとんどなかった。本研究では光合成により吸収された炭素が木質細胞壁として堆積される過程について情報を得ることができた。また得られた知見は、木質バイオマス生産を制御する技術の確立や、高いCO2固定能力をもつ等の優れた特性を有する樹木を設計する上でも有用であるといえる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019 2018

All Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Presentation] ポプラ引張あて材形成開始時における木部形成2022

    • Author(s)
      竹内美由紀
    • Organizer
      第72回 日本木材学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 13CO2パルスラベリングと同位体分布解析によるポプラ引張あて材形成過程の研究2021

    • Author(s)
      石田傑、齋藤継之、竹内美由紀、則定真利子、堀川祥生
    • Organizer
      第71回 日本木材学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 樹木木部細胞壁の形成を観察する2021

    • Author(s)
      竹内美由紀, 則定真利子, 磯貝明
    • Organizer
      第132回 日本森林学会大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] ポプラ木部細胞壁形成における二酸化炭素と水の利用2020

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      第70回木材学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Incorporation of tracer 13C to developing xylem of poplar2019

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      Cell wall meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ポプラ木部細胞壁形成における光合成産物の利用2019

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Tracing photosynthetic carbon distribution during xylem formation2019

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      22th International Conference on Secondary Ion Mass Spectrometry (SIMS22)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 13CO2ラベリングを用いたポプラ木部における光合成産物の分布解析2019

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      第69回木材学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Imaging mass spectrometry analysis of photosynthetic products in plants2018

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      SISS20
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 同位体パルスラベリングを用いた樹木木部形成過程の解析2018

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Tracing photosynthetic carbon in wood after 13CO2 labelling2018

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      2018 Joint Convention SWST & JWRS
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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