Evaluation of genetic basis for life history polymorphism in masu salmon: based on genetics related to metabolism

• Project/Area Number: 18K05796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Yamamoto Toshiaki 日本獣医生命科学大学, 獣医学部, 准教授 (30409255)
• Co-Investigator(Kenkyū-buntansha): 北西 滋 大分大学, 理工学部, 准教授 (90552456)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 遺伝子発現 / スモルト / 生活史多型 / RNA-seq / RNAseq / リアルタイムPCR / 成熟 / 遺伝子発現量 / サクラマス / 次世代シーケンス / RNA発現 / 残留型 / 降海型 / 遺伝的要因 / 代謝量 / 代謝関連遺伝子 / 遺伝的基盤 / 生活史分岐

Outline of Final Research Achievements

In this study, in order to clarify a genetic basis for the life history polymorphism in masu salmon Oncorhynchus masou, I conducted the GRAS-Di analysis and RNA-seq analysis using a next-generation sequence. As a result, some candidate genes in conjunction with the smolting were able to detect. On the other hand, the candidate genes in conjunction with the sexual maturation as the resident form was complicated, and it was suggested that many hereditary domains was related to determine whether individuals are the maturation. Besides, I crossbred using some parents of the different life history and tested gene expression of offspring based on half-sib in each time. As a result, the expression of the candidate genes in conjunction with the smolting showed that it did not express at least until the early stage of life history.

Academic Significance and Societal Importance of the Research Achievements

生活史多型は様々な動物群においてみられる現象であり、これまでに多くの研究者を魅了してきたが、それら生活史に関連した遺伝子は未だ十分に解明されていない。本研究では、スモルト期に関連しいる遺伝子が限定的にすることが出来たことは、初めての生活史多型に関連した遺伝子の解明につながると考えられることから、非常に学術的な意義は高い。さらには、水産資源としても重要なサケ科魚類では、関連遺伝子が明らかになった場合においては、効率的な資源作成にもつながることが期待されるため、社会的なインパクトも非常に大きいと考えらる。

Research Products

• [Journal Article] Effect of parental phenotype on dispersal, growth and maturation of offspring in wild masu salmon (Oncorhynchus masou) (2021)
  • Author(s): Yamamoto Toshiaki、Kitanishi Shigeru、Metcalfe Neil B.
  • Journal Title: Evolutionary Ecology Volume: 35 Pages: 253-269
  • Peer Reviewed
• [Journal Article] Links between paternal life history and offspring metabolic rate and body size during early life in masu salmon Oncorhynchus masou (2021)
  • Author(s): Yamamoto Toshiaki, Kitanishi Shigeru, Masato Sato, Yagisawa Masaru, Kishi Daisuke
  • Journal Title: Ecology of Freshwater Fish Volume: -
  • Peer Reviewed
• [Journal Article] 尾瀬ヶ原の下ヨサク沢に生息するイワナSalvelinus leucomaenlsの遺伝的特性について (2021)
  • Author(s): 山本俊昭、藤原英史、萩原富司、野原精一
  • Journal Title: 陸水学会誌 Volume: -
  • Peer Reviewed
• [Journal Article] Examining multiple paternity in the raccon dog (Nyctereutes procyonoides) in Japan using microsatellite analysis. (2020)
  • Author(s): Sugiura N., Ochiai K., Yamamoto T., Kato T., KawamotoY., Omi T., and Hayama S.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 82 Pages: 479-482
  • Peer Reviewed
• [Journal Article] Examining multiple paternity in the raccoon dog (<i>Nyctereutes procyonoides</i>) in Japan using microsatellite analysis (2020)
  • Author(s): SUGIURA Natsuko、OCHIAI Kazuhiko、YAMAMOTO Toshiaki、KATO Takuya、KAWAMOTO Yoshi、OMI Toshinori、HAYAMA Shin-ichi
  • Journal Title: Journal of Veterinary Medical Science Volume: 82 Issue: 4 Pages: 479-482
  • DOI: 10.1292/jvms.19-0655
  • NAID: 130007829871
  • ISSN: 0916-7250, 1347-7439
• [Presentation] サクラマスにおける早熟雄の閾値は集団構造で変化するのか？ (2020)
  • Author(s): 山本俊昭
  • Organizer: 第20回東日本魚類研究会
• [Presentation] サクラマス3亜種は遺伝的にどこまで違うのか：次世代シーケンスによる試み (2019)
  • Author(s): 山本俊昭
  • Organizer: 東日本魚類研究会
• [Book] 動物看護の教科書 (2020)
  • Author(s): 分担）山本俊昭
  • Total Pages: 20
  • Publisher: 緑書房
  • ISBN: 9784895314145