| Project/Area Number |
18K05805
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40030:Aquatic bioproduction science-related
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| Research Institution | Fisheries Research and Education Agency |
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Principal Investigator |
Shimahara Yoshiko 国立研究開発法人水産研究・教育機構, 水産技術研究所(南勢), 主任研究員 (50570115)
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| Co-Investigator(Kenkyū-buntansha) |
西木 一生 宮崎大学, 農学部, 助教 (70817048)
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| Project Period (FY) |
2018-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ノカルジア症 / Nocardia seriolae / ブリ / SOD / カタラーゼ / 食細胞 / マクロファージ |
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| Outline of Final Research Achievements |
Intracellular bacteria can enter and survive in host cells through a variety of strategies. Nocardia seriolae, which is a causative agent of nocardiosis in yellowtail, is known as intracellular bacteria and is thought to utilize phagocytes to spread infection in infected fish. Although SOD and catalase genes, which are thought to be involved in the detoxification of reactive oxygen species, has been confirmed to be present in N. seriolae genome, it is unknown whether these genes are expressed during infection. In this study, RT-qPCR for each gene was newly constructed and the expressions of these genes were determined with artificially infected fish. Both SOD and catalase genes were found to be expressed in the spleen of the infected fish. Resistance to host bactericidal activity by SOD and catalase was suggested to play an important role in N. seriolae infection.
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| Academic Significance and Societal Importance of the Research Achievements |
活魚類の細菌性疾病では、類結節症やエドワジエラ症などが細胞内寄生菌による感染症として知られており、宿主の殺菌作用に対しどのように抵抗しているのか知見がいくつか得られている。ノカルジア菌については、ゲノム上にSODやカタラーゼ遺伝子の存在が確認されているものの、それらの発現をモニタリングした研究は見当たらない。本研究により、ノカルジア菌のSODとカタラーゼが宿主の殺菌作用に抵抗する役割を果たしていることが示唆された。本菌の病原性メカニズム解明の基盤となる知見が得られたことは、細菌学的な面はもちろん、ブリ類で被害の大きいノカルジア症対策を構築する上でも大きな意義を持つといえる。
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