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Isolation of causative gene in target genomic regions using genome editing in ayu, Plecoglossus altivelis

Research Project

Project/Area Number 18K05816
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Nakamoto Masatoshi  東京海洋大学, 学術研究院, 博士研究員 (80447721)

Co-Investigator(Kenkyū-buntansha) 坂本 崇  東京海洋大学, 学術研究院, 教授 (40313390)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords水産育種 / ゲノム編集 / アユ / 性決定 / 性分化 / ターゲットシークエンス
Outline of Final Research Achievements

Ayu (or sweetfish), Plecoglossus altivelis, is widely distributed in East Asia. Ayu belongs to the superorder Stomiati and the order Osmeriformes. Stomiati is phylogenetically classified as sister group of Neoteleostei, the largest clade of bony fish including medaka, tuna and cod. Thus, ayu holds an important position in the fish tree of life. In Japan, female ayu are more economically important than males as food. Therefore, there is a need for genetic sex identification of ayu in aquaculture. We identified sex-associated loci by a genome-wide association study using wild populations. Expression analysis for the candidate gene of Y-linked sex determining gene reveled that mRNA of candidate gene was expressed in supporting cells surrounding germ cells in male undifferentiated gonad. Loss-of-function mutation for the candidate gene induced male to female sex reversal. Taken together, these results indicate that the candidate gene on the Y chromosome determines genetic sex.

Academic Significance and Societal Importance of the Research Achievements

水産重要種であるアユの養殖においては子持ちアユとしてメスが珍重されており、全雌生産のために遺伝的性判別のニーズが存在する。アユはXX-XY型の性決定様式でありY染色体上の性決定遺伝子によって性が決まると考えられている。本研究によりアユの性決定遺伝子が同定され、確実に遺伝的性を判別できる遺伝マーカーが開発されたことにより、遺伝的性を養殖の初期段階で判別することでより多くの雌を生産することが可能となる。またXX個体に対してホルモン処理等の性転換処理を行いXX雄を作出することで全雌生産ができるようになる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2021 2020 2019 2018

All Presentation (3 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] アユの性決定遺伝子候補の発現解析2021

    • Author(s)
      中本正俊, 坂本崇
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ゲノムワイド相関解析によるアユ性決定遺伝子座の探索2019

    • Author(s)
      中本正俊, 内野翼, Guiguen Yann, 坂本崇
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] GENOME SEQUENCING AND MAPPING OF SEX-DETERMINING LOCUS IN AYU PLECOGLOSSUS ALTIVELIS2018

    • Author(s)
      Nakamoto Masatoshi, Uchino Tsubasa, Guiguen Yann, Sakamoto Takashi
    • Organizer
      International Symposium of Genetics in Aquaculture XIII
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] アユ類の性識別方法2020

    • Inventor(s)
      坂本崇 中本正俊
    • Industrial Property Rights Holder
      坂本崇 中本正俊
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-203939
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-12-28  

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