• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the mechanism of silica cell wall formation by monitoring the expression dynamics of the cell wall formation-related proteins

Research Project

Project/Area Number 18K05818
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionOkayama University

Principal Investigator

Nemoto Michiko  岡山大学, 環境生命科学研究科, 助教 (30625926)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords珪藻 / バイオミネラリゼーション / RNA-seq解析 / 形質転換 / 同調培養
Outline of Final Research Achievements

The purpose of this study is to get insight into the function of silica cell wall formation-related proteins by analyzing the expression pattern of these transcripts during the silica cell wall formation in diatoms. The comprehensive comparative analysis of eight diatom species identified a number of diatom-specific genes that have likely associations with silica cell wall formation, including a previously unrecognized SET domain protein methyltransferase family. Subsequent proteomic analyses revealed the presence of specific silica cell wall-associated proteins in Nitzschia palea. Expression analysis showed that the expression of the diatom-specific SET domain proteins and silica cell wall-associated proteins were upregulated in response to silicon, suggesting that these genes play roles in silica cell wall formation.

Academic Significance and Societal Importance of the Research Achievements

これまで、珪藻の被殻形成関連タンパク質として同定・報告されたタンパク質はジメチル化およびトリメチル化されたリジン残基を持つことが知られており、シリカ形成におけるメチル化修飾の重要性が示唆されていたが、珪藻の被殻形成関連タンパク質をメチル化する酵素は見つかっていなかった。本研究により同定された珪藻特異的新規メチルトランスフェラーゼは、被殻形成関連タンパク質をメチル化することで、珪藻のシリカ形成を制御するタンパク質であることが示唆された。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (28 results)

All 2021 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 3 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results)

  • [Journal Article] Multiple mutations in RNA polymerase β-subunit gene (rpoB) in Streptomyces incarnatus NRRL8089 enhance production of antiviral antibiotic sinefungin: modeling rif cluster region by density functional theory2021

    • Author(s)
      Ogawa Saori、Shimidzu Hitomi、Fukuda Koji、Tsunekawa Naoki、Hirano Toshiyuki、Sato Fumitoshi、Yura Kei、Hasunuma Tomohisa、Ochi Kozo、Yamamoto Michio、Sakamoto Wataru、Hashimoto Kentaro、Ogata Hiroyuki、Kanao Tadayoshi、Nemoto Michiko、Inagaki Kenji、Tamura Takashi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 85 Issue: 5 Pages: 1275-1282

    • DOI

      10.1093/bbb/zbab011

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of long-term antiretroviral therapy on gut and oral microbiotas in HIV-1-infected patients2021

    • Author(s)
      Imahashi Mayumi、Ode Hirotaka、Kobayashi Ayumi、Nemoto Michiko、Matsuda Masakazu、Hashiba Chieko、Hamano Akiko、Nakata Yoshihiro、Mori Mikiko、Seko Kento、Nakahata Masashi、Kogure Ayumi、Tanaka Yasuhito、Sugiura Wataru、Yokomaku Yoshiyuki、Iwatani Yasumasa
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 960-960

    • DOI

      10.1038/s41598-020-80247-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Radular Stylus of Cryptochiton Stelleri: A Multifunctional Lightweight and Flexible Fiber-Reinforced Composite2020

    • Author(s)
      Anna Pohl, Steven A. Herrera, David Restrepo, Ryo Negishi, Jae-Young Jung, Chris Salinas, Richard Wuhrer, Tomoko Yoshino, Joanna McKittrick, Atsushi Arakaki, Michiko Nemoto, Pablo Zavattieri, David Kisailus
    • Journal Title

      J Mech Behav Biomed Mater

      Volume: 111 Pages: 103991-103991

    • DOI

      10.1016/j.jmbbm.2020.103991

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparative gene analysis focused on silica cell wall formation: Identification of diatom-specific SET domain protein methyltransferases2020

    • Author(s)
      Nemoto, M., Iwaki, S., Moriya, H., Monden, Y., Tamura, T., Inagaki, K., Mayama, S. and Obuse K.
    • Journal Title

      Marine Biotechnology

      Volume: - Issue: 4 Pages: 551-563

    • DOI

      10.1007/s10126-020-09976-1

    • NAID

      120007045009

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antiviral effect of sinefungin on in vitro growth of feline herpesvirus type 12019

    • Author(s)
      Kuroda Yudai、Yamagata Haruka、Nemoto Michiko、Inagaki Kenji、Tamura Takashi、Maeda Ken
    • Journal Title

      The Journal of Antibiotics

      Volume: 72 Issue: 12 Pages: 981-985

    • DOI

      10.1038/s41429-019-0234-4

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri2019

    • Author(s)
      Nemoto Michiko、Ren Dongni、Herrera Steven、Pan Songqin、Tamura Takashi、Inagaki Kenji、Kisailus David
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-018-37839-2

    • NAID

      120006727115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Redox-tuning of oxidizing disulfide oxidoreductase generates a potent disulfide isomerase2019

    • Author(s)
      Sutoh Shinya、Uemura Yuko、Yamaguchi Yuko、Kiyotou Asako、Sugihara Rena、Nagayasu Makiko、Kurokawa Mihoko、Ito Koreaki、Tsunekawa Naoki、Nemoto Michiko、Inagaki Kenji、Tamura Takashi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

      Volume: 1867 Issue: 3 Pages: 194-201

    • DOI

      10.1016/j.bbapap.2018.12.005

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Compound heterozygous TYK2 mutations underlie primary immunodeficiency with T-cell lymphopenia2018

    • Author(s)
      Nemoto Michiko、Hattori Hiroyoshi、Maeda Naoko、Akita Nobuhiro、Muramatsu Hideki、Moritani Suzuko、Kawasaki Tomonori、Maejima Masami、Ode Hirotaka、Hachiya Atsuko、Sugiura Wataru、Yokomaku Yoshiyuki、Horibe Keizo、Iwatani Yasumasa
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 6956-6956

    • DOI

      10.1038/s41598-018-25260-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒザラガイ類のトランスクリプトーム比較による磁鉄鉱形成関連因子の探索2021

    • Author(s)
      岡田航輝
    • Organizer
      日本農芸化学会中四国支部第58回講演会(例会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒザラガイの磁鉄鉱歯に特異的な新奇タンパク質RTMP1の機能解析2021

    • Author(s)
      楢原優佳
    • Organizer
      日本農芸化学会中四国支部第58回講演会(例会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 珪藻のシリカ被殻局在タンパク質の同定および比較解析2020

    • Author(s)
      根本理子
    • Organizer
      第43回 日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 珪藻種間の遺伝子比較解析およびプロテオーム解析に基づくシリカ細胞壁形成関連タンパク質の同定2020

    • Author(s)
      岩城沙弥子, 小布施祈織, 田村隆, 稲垣賢二, 根本理子
    • Organizer
      日本農芸化学会中四国支部第56回講演会(例会)
    • Related Report
      2019 Research-status Report
  • [Presentation] Nitzschia属珪藻を用いた被殻形成関連タンパク質の解析2019

    • Author(s)
      根本理子、岩城沙弥子、岡田航輝、田村隆、 稲垣賢二、真山茂樹、小布施祈織
    • Organizer
      第6回分子珪藻研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] RNA-seqを利用したバイオミネラリゼーション機構の解析2019

    • Author(s)
      根本理子
    • Organizer
      日本農芸化学会中四国支部 第30回若手シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 非モデル珪藻のトランスクリプトーム解析およびプロテオーム解析に基づく被殻形成関連タンパク質の同定2019

    • Author(s)
      岩城 沙弥子, 小布施 祈織, 田村 隆, 稲垣 賢二, 真山 茂樹, 根本 理子
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Transcriptome and proteome analysis of non-model diatom species toward understanding of silica biomineralization2019

    • Author(s)
      Michiko Nemoto, Sayako Iwaki, Takashi Tamura, Kenji Inagaki, Shigeki Mayama, Kiori Obuse
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expression and characterization of recombinant radular teeth matrix protein 1 (RTMP1) from Cryptochiton stelleri2019

    • Author(s)
      Yuka Narahara, Takashi Tamura, Kenji Inagaki, Kenji Okoshi, David Kisailus, Hisao Moriya, Michiko Nemoto
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative transcriptomics of three diatom species focused on silica biomineralization2019

    • Author(s)
      Sayako Iwaki, Kiori Obuse, Takashi Tamura, Kenji Inagaki, Shigeki Mayama, Michiko Nemoto
    • Organizer
      The molecular life of diatoms
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 3種の珪藻の比較トランスクリプトーム解析に基づく細胞壁形成関連タンパク質の同定2019

    • Author(s)
      岩城沙弥子、小布施祈織、田村隆、稲垣賢二、根本理子
    • Organizer
      おかやまバイオアクティブ研究会 第55回シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] 生体鉱物の形成に関わるタンパク質の探索2019

    • Author(s)
      根本理子
    • Organizer
      日本農芸化学会中四国支部 第29回若手シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ゲノム未知の珪藻からの遺伝子転写調節領域の取得及びそれを利用した形質転換用ベクターの構築2019

    • Author(s)
      白石幸音,田村隆,稲垣賢二,真山茂樹,根本理子
    • Organizer
      日本農芸化学会中四国支部第53回講演会(例会)
    • Related Report
      2018 Research-status Report
  • [Presentation] 珪藻Nitzschia paleaの被殻形成関連タンパク質の同定2018

    • Author(s)
      岩城沙弥子、田村隆、稲垣賢二、根本理子
    • Organizer
      第20回マリンバイオテクノロジー学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] オミックス解析に基づくオオバンヒザラガイの歯舌形成関連タンパク質の同定2018

    • Author(s)
      根本理子、Dongni Ren、Steven Herrera、田村隆、稲垣賢二、David Kisailus
    • Organizer
      第20回マリンバイオテクノロジー学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Identification of proteins related to tooth mineralization in Cryptochiton stelleri by integrated transcriptomic and proteomic analysis2018

    • Author(s)
      Michiko Nemoto, Dongni Ren, Steven Herrera, Takashi Tamura, Kenji Inagaki and David Kisailus
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 異なる珪藻種間の遺伝子比較解析およびプロテオーム解析に基づく珪藻の被殻形成因子の探索2018

    • Author(s)
      根本理子、岩城沙弥子、田村隆、 稲垣賢二、小布施祈織
    • Organizer
      第5回分子珪藻研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Identification of proteins involved in tooth biomineralization in the giant Pacific chiton by integrated transcriptomic and proteomic analysis2018

    • Author(s)
      Michiko Nemoto, Dongni Ren, Steven Herrera, Takashi Tamura, Kenji Inagaki and David Kisailus
    • Organizer
      Bioactive Okayama 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒザラガイ歯舌の磁鉄鉱形成メカニズム2018

    • Author(s)
      根本理子、David Kisailus
    • Organizer
      第13回バイオミネラリゼーションワークショップ
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] Biological Magnetic Materials and Applications (Chapter3を分担執筆)2018

    • Author(s)
      Kisailus, D. and Nemoto, M.
    • Total Pages
      199
    • Publisher
      Springer Singapore
    • ISBN
      9789811080685
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi