| Project/Area Number |
18K05818
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40040:Aquatic life science-related
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| Research Institution | Okayama University |
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Principal Investigator |
Nemoto Michiko 岡山大学, 環境生命科学研究科, 助教 (30625926)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 珪藻 / バイオミネラリゼーション / RNA-seq解析 / 形質転換 / 同調培養 |
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| Outline of Final Research Achievements |
The purpose of this study is to get insight into the function of silica cell wall formation-related proteins by analyzing the expression pattern of these transcripts during the silica cell wall formation in diatoms. The comprehensive comparative analysis of eight diatom species identified a number of diatom-specific genes that have likely associations with silica cell wall formation, including a previously unrecognized SET domain protein methyltransferase family. Subsequent proteomic analyses revealed the presence of specific silica cell wall-associated proteins in Nitzschia palea. Expression analysis showed that the expression of the diatom-specific SET domain proteins and silica cell wall-associated proteins were upregulated in response to silicon, suggesting that these genes play roles in silica cell wall formation.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、珪藻の被殻形成関連タンパク質として同定・報告されたタンパク質はジメチル化およびトリメチル化されたリジン残基を持つことが知られており、シリカ形成におけるメチル化修飾の重要性が示唆されていたが、珪藻の被殻形成関連タンパク質をメチル化する酵素は見つかっていなかった。本研究により同定された珪藻特異的新規メチルトランスフェラーゼは、被殻形成関連タンパク質をメチル化することで、珪藻のシリカ形成を制御するタンパク質であることが示唆された。
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