• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Crustacean insulin-like peptides and their relationship to reproduction and nutrition

Research Project

Project/Area Number 18K05819
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionMie University

Principal Investigator

Tsutsui Naoaki  三重大学, 生物資源学研究科, 准教授 (00643785)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsインスリン様ペプチド / クルマエビ / 生殖 / 栄養 / 甲殻類 / 発現解析
Outline of Final Research Achievements

In this study, three novel insulin-like peptides [ILP-1, ILP-2, and gonadulin (GON)] were found by transcriptome analysis in the kuruma prawn. Tissue-specific expression analyses on adults revealed sexual dimorphism and site-specific expression of these genes in the gonads: ILP1 in the ovary, ILP2 in the testis, and GON in the vas deferens. In contrast, no clear sex differences in gene expression were identified at least until 74 days after post-larval metamorphosis. Synthesized ILP1 and ILP2 had no effect on vitellogenin gene expression in the incubated ovary or hepatopancreas fragments.

Academic Significance and Societal Importance of the Research Achievements

インスリンファミリーは多機能なペプチドであり、特に脱皮動物のグループではエネルギーと生殖とをリンクする制御系を構成していると考えられる。本研究では、クルマエビで見つかった3種類の新規インスリン様ペプチドが、雌雄の生殖腺で特徴的な発現様式を示すことを示した。生殖や栄養状態の制御に関わる直接的な知見は得られなかったものの、血中グルコース濃度との関連など、今後栄養と生殖との関係をより深く理解するための基礎的知見を集積することができた。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Multiple insulin-like peptides in the gonads of the kuruma prawn Marsupenaeus japonicus2022

    • Author(s)
      Tsutsui Naoaki、Yamane Fumihiro、Kakinuma Makoto、Yoshimatsu Takao
    • Journal Title

      Fisheries Science

      Volume: - Issue: 3 Pages: 387-396

    • DOI

      10.1007/s12562-022-01596-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptomic Analysis of the Kuruma Prawn Marsupenaeus japonicus Reveals Possible Peripheral Regulation of the Ovary2020

    • Author(s)
      Tsutsui Naoaki、Kobayashi Yasuhisa、Izumikawa Kouichi、Sakamoto Tatsuya
    • Journal Title

      Frontiers in Endocrinology

      Volume: 11 Pages: 541-541

    • DOI

      10.3389/fendo.2020.00541

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of different microalgal diets on the growth performance of Japanese pearl oyster Pinctada fucata martensii broodstock2020

    • Author(s)
      Yamamoto Satoshi、Tsutsui Tsutomu、Saguchi Tomoyuki、Tsutsui Naoaki、Yoshimatsu Takao
    • Journal Title

      Aquaculture Research

      Volume: 51 Issue: 11 Pages: 4809-4813

    • DOI

      10.1111/are.14785

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of salinity change and exposure time on the egg stages of two abalone species Haliotis discus discus and H. gigantea2020

    • Author(s)
      Albert V. Manuel, Phan Thi Cam Tu, Naoaki Tsutsui, Takao Yoshimatsu
    • Journal Title

      Fisheries Science

      Volume: 86 Issue: 1 Pages: 27-33

    • DOI

      10.1007/s12562-019-01366-4

    • NAID

      40022128316

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 眼柄切除が小型雄クルマエビのインスリン様造雄腺因子の産生亢進を通じて精子形成を促進する可能性2022

    • Author(s)
      古川雄裕、奥村卓二、山根史裕、筒井直昭
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 眼柄切除が雄クルマエビの生殖内分泌軸へ及ぼす影響2021

    • Author(s)
      古川雄裕、奥村卓二、山根史裕、筒井直昭
    • Organizer
      第45回日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] クルマエビMarsupenaeus japonicusにおけるインスリン様ペプチド遺伝子の探索と発現解析2021

    • Author(s)
      筒井直昭
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] A transcriptomic search for genes encoding putative hormones expressed in the prawn ovary2019

    • Author(s)
      Naoaki Tsutsui, Tatsuya Sakamoto
    • Organizer
      The 12th International Marine Biotechnology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] クルマエビの肝膵臓で発現する新規ビテロジェニン様遺伝子2019

    • Author(s)
      筒井直昭・吉松隆夫・水藤勝喜・坂本竜哉
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] クルマエビに見出された2種のインスリン様ペプチド2018

    • Author(s)
      筒井直昭
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi