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Identification of genes responsible for white shell phenotype in the pearl oyster and development of genome editing technology

Research Project

Project/Area Number 18K05834
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

Takeuchi Takeshi  沖縄科学技術大学院大学, マリンゲノミックスユニット, スタッフサイエンティスト (60599231)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsアコヤガイ / 真珠養殖 / ゲノム編集 / トランスクリプトーム / プロテオーム / ゲノム
Outline of Final Research Achievements

Cultured pearls produced by pearl oysters are one of the most important fishery resources in Japan. There is a known mutation in the pearl oysters that produce white shells, and the mutants are used to produce pure white cultured pearls. In this study, we constructed a high-precision genome assembly and analyzed the transcriptome and proteome of the pearl oyster to identify the genes responsible for the white shell phenotype. We identified several candidate genes responsible for the white shell mutation based on these results.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ゲノム編集に必要な、高分子をアコヤガイ生体に導入するための様々な手法を試行した。実験によって得られた知見は今後のアコヤガイゲノム編集技術の確立にむけて有用な情報となる。また、アコヤガイ外套膜における網羅的な遺伝子発現データ、産出されるタンパク質データや、染色体スケールの高精度ゲノムデータは、アコヤガイのあらゆる生物学的機能を研究する上で極めて重要なデータベースとなる。これらのデータベースはインターネットで公開し研究コミュニティに提供することで、アコヤガイおよび軟体動物研究の活性化に役立てる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (8 results) (of which Invited: 4 results)

  • [Journal Article] The ‘Shellome’ of the Crocus Clam Tridacna crocea Emphasizes Essential Components of Mollusk Shell Biomineralization2021

    • Author(s)
      Takeshi Takeuchi, Manabu Fujie, Ryo Koyanagi, Laurent Plasseraud, Isabelle Ziegler-Devin, Nicolas Brosse, Cedric Broussard, Noriyuki Satoh, Frederic Marin
    • Journal Title

      Frontiers in Genetics

      Volume: 12 Pages: 1-16

    • DOI

      10.3389/fgene.2021.674539

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Whole-Genome Sequencing Highlights Conservative Genomic Strategies of a Stress-Tolerant, Long-Lived Scleractinian Coral, Porites australiensis Vaughan, 19182021

    • Author(s)
      Chuya Shinzato, Takeshi Takeuchi, Yuki Yoshioka, Ipputa Tada, Miyuki Kanda, Cedric Broussard, Akira Iguchi, Makoto Kusakabe, Frederic Marin, Noriyuki Satoh, Mayuri Inoue
    • Journal Title

      Genome Biology and Evolution

      Volume: 13 Issue: 12 Pages: 1-16

    • DOI

      10.1093/gbe/evab270

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome-wide SNP genotyping reveals hidden population structure of an acroporid species at a subtropical coral island: Implications for coral restoration2021

    • Author(s)
      Yuna Zayasu, Takeshi Takeuchi, Tomofumi Nagata, Megumi Kanai, Manabu Fujie, Mayumi Kawamitsu, Wakana Chinen, Chuya Shinzato, Noriyuki Satoh
    • Journal Title

      Aquatic Conservation: Marine and Freshwater Ecosystems

      Volume: 31 Issue: 9 Pages: 2429-2439

    • DOI

      10.1002/aqc.3626

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Divergent northern and southern populations and demographic history of the pearl oyster in the western Pacific revealed with genomic SNPs2020

    • Author(s)
      Takeuchi Takeshi、Masaoka Tetsuji、Aoki Hideo、Koyanagi Ryo、Fujie Manabu、Satoh Noriyuki
    • Journal Title

      Evolutionary Applications

      Volume: 13 Issue: 4 Pages: 837-853

    • DOI

      10.1111/eva.12905

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 二倍体アコヤガイゲノムのハプロタイプ別アセンブリ2022

    • Author(s)
      竹内猛, 鈴木 慶彦, 渡部 終五, 永井 清仁, 正岡 哲治, 藤江 学, 川満 真由美, 佐藤 矩行, Eugene W. Myers
    • Organizer
      核酸研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 貝殻に色素を蓄積しないアコヤガイ突然変異体の原因遺伝子の探索2021

    • Author(s)
      堀田大樹、木下滋晴、吉武和敏、浅川修一、竹内猛、佐藤矩行、前山薫、永井清仁、渡部終五
    • Organizer
      日本水産学会春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヒメジャコ(Tridacna crocea)貝殻プロテオーム解析から明らかになった貝殻基質タンパク質の保存性と多様性2020

    • Author(s)
      竹内 猛
    • Organizer
      バイオミネラリゼーションワークショップ
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] アコヤガイの全ゲノム解読と水産育種への利用2020

    • Author(s)
      竹内猛, 正岡哲治, 青木秀夫, 永井清仁, 佐藤矩行
    • Organizer
      日本水産学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] アコヤガイの全ゲノム情報を利用した貝殻白色化原因遺伝子の同定2020

    • Author(s)
      竹内猛, 藤江学, 小柳亮, 小田原和史, 吉武和敏, 木下滋晴, 浅川修一, 渡部終五, 佐藤矩行
    • Organizer
      日本水産学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 貝殻に色素を蓄積しないアコヤガイ変異体の原因遺伝子探索2020

    • Author(s)
      堀田大樹, 木下滋晴, 吉武和敏, 浅川修一, 竹内猛, 佐藤矩行, 前山薫, 永井清仁, 渡部終五
    • Organizer
      日本水産学会
    • Related Report
      2019 Research-status Report
  • [Presentation] アコヤガイ真珠品質関連遺伝子の探索2020

    • Author(s)
      木下滋晴, 堀田大樹, 篠原幹拓, 吉武和敏, 浅川修一, 舩原大輔, 柿沼誠, 竹内猛, 佐藤矩行, 淡路雅彦, 前山薫, 永井清仁, 渡部終五
    • Organizer
      日本水産学会
    • Related Report
      2019 Research-status Report
  • [Presentation] The pearl oyster genome: evolutionary origin of the shells and pearls2019

    • Author(s)
      Takeshi Takeuchi
    • Organizer
      The Symposium of Recent Activity in Marine Genome Biology
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2018-04-23   Modified: 2023-01-30  

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