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Development of non-thermal plasma sterilization technology for hydroponic nutrient solution for measures against wilt disease in a plant factory

Research Project

Project/Area Number 18K05902
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionNagoya Institute of Technology

Principal Investigator

Yasui Shinji  名古屋工業大学, 工学(系)研究科(研究院), 教授 (30371561)

Co-Investigator(Kenkyū-buntansha) 庄子 和博  一般財団法人電力中央研究所, エネルギーイノベーション創発センター, 上席研究員 (10371527)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords大気圧プラズマ / 殺菌 / 培養液 / 液中プラズマ / 植物工場 / 萎凋病 / フザリウム / ピシウム / OHラジカル / 水耕栽培 / プラズマ / 非熱プラズマ
Outline of Final Research Achievements

We have developed a sterilization technology using low temperature atmospheric pressure plasma of a nutrient solution for nutrient thin film hydroponics in a plant factory. We have firstly shown that Fusarium oxysporum and Pythium aphanidermatum, which are the pathogenic bacteria of wilt disease in thin-film hydroponics such as spinach in summer, can be completely sterilized by plasma irradiating to the nutrient solution. The sterilization mechanism was elucidated by various control experiments, and it was clarified that the OH radical generated in the solution is an extremely large sterilization factor. Bacterial contamination of the culture medium invades from the roots with human intervention during planting of seedlings. In a demonstration experiment using spinach hydroponics, it was clarified that wilt disease can be controlled by short-term plasma irradiation of about 5 minutes both before and after planting seedlings.

Academic Significance and Societal Importance of the Research Achievements

培養液中へのプラズマの直接照射により,薄膜水耕栽培での萎凋病の病原菌であるフザリウム胞子とピシウム菌を殺菌できることを世界で初めて実証した。特に,植物工場では培養液の成分が変質しないことが重要であり,この技術はアルゴンプラズマの照射により,溶液中のH2Oから殺菌力の強いOHラジカルを生成し,殺菌に寄与した後は,即座にOHラジカルが消滅する利点がある。植物工場水耕栽培で大きな課題となっている病原菌の侵入に対して,初めて期待に沿える技術となる。病原菌の侵入は苗の定植時に生じるが,その前後に数分程度のプラズマ照射で防除できることも世界で初めて示した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2020 2019 2018 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Basic Evaluation of Fusarium Wilt Prevention of Spinach by Plasma Irradiation in Solution2020

    • Author(s)
      坂本源起,伊藤大樹,安井晋示,寺添斉,庄子和博
    • Journal Title

      Shokubutsu Kankyo Kogaku

      Volume: 32 Issue: 1 Pages: 37-43

    • DOI

      10.2525/shita.32.37

    • NAID

      130007804581

    • ISSN
      1880-2028, 1880-3563
    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sterilization of <I>Pythium aphanidermatum</I> Zoospores Using Surface Discharge Plasma in Solution2020

    • Author(s)
      平原大地,坂本源起,安井晋示,景山 幸二
    • Journal Title

      Shokubutsu Kankyo Kogaku

      Volume: 32 Issue: 2 Pages: 115-121

    • DOI

      10.2525/shita.32.115

    • NAID

      130007850386

    • ISSN
      1880-2028, 1880-3563
    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 萎凋病対策に向けた培養液のプラズマ殺菌技術2019

    • Author(s)
      安井晋示
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 38-40

    • NAID

      40021926483

    • Related Report
      2018 Research-status Report
  • [Presentation] 沿面放電液中プラズマ殺菌におけるOH ラジカルの影響検証2020

    • Author(s)
      平原大地,安井晋示,景山幸二
    • Organizer
      電気学会全国大会
    • Related Report
      2020 Annual Research Report 2019 Research-status Report
  • [Presentation] 沿面放電液中プラズマの Fusarium oxysporum殺菌特性に対する極性効果2019

    • Author(s)
      平原大地,坂本源起,LE DINH TUNG,安井晋示,庄子和博,寺添 斉
    • Organizer
      電気学会 放電・プラズマ・パルスパワー研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 沿面放電を用いた液中プラズマによるOHラジカルの生成特性2019

    • Author(s)
      LE DINH TUNG,伊藤大樹,安井晋示
    • Organizer
      電気学会全国大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 沿面放電液中プラズマによるフザリウム萎凋病防除効果の検証2019

    • Author(s)
      坂本源起,伊藤大樹,安井晋示,寺添斉,庄子和博
    • Organizer
      電気学会全国大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 沿面放電液中プラズマのPythium属菌遊走子への殺菌効果2019

    • Author(s)
      平原大地,坂本源起,安井晋示,景山幸二
    • Organizer
      電気学会全国大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 沿面放電による液中プラズマの放電特性2018

    • Author(s)
      LE DINH TUNG、伊藤 大樹、安井 晋示
    • Organizer
      電気学会東海支部連合大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 沿面放電液中により発生させたOHラジカルのスペクトル特性評価2018

    • Author(s)
      伊藤大樹,LE DINH TUNG,安井晋示
    • Organizer
      電気学会プラズマ・パルスパワー・放電合同研究会
    • Related Report
      2018 Research-status Report
  • [Remarks] 水中沿面放電プラズマによる培養液の殺菌や植物の生育促進

    • URL

      http://yasui-lab.web.nitech.ac.jp/research/

    • Related Report
      2020 Annual Research Report 2018 Research-status Report
  • [Remarks] 水中沿面放電プラズマによる培養液の殺菌や植物の生育促進

    • URL

      http://yasui-lab.web.nitech.ac.jp/

    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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