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Study on the sensing mechanism of nitrogen dioxide through PIF4 protein regulation

Research Project

Project/Area Number 18K05926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41050:Environmental agriculture-related
Research InstitutionHiroshima University

Principal Investigator

Takahashi Misa  広島大学, 統合生命科学研究科(理), 助教 (10294513)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords二酸化窒素 / シロイヌナズナ / PIF4 / 胚軸伸長 / 環境応答 / 胚軸伸張 / ChIP解析 / バイオマス
Outline of Final Research Achievements

Atmospheric nitrogen dioxide (NO2) at ambient concentrations (10~50 ppb) is as a novel positive growth regulator for plants. NO2 accelerates cell proliferation and cell enlargement to nearly double organ size and shoot biomass in a variety of species including Arabidopsis. NO2 also accelerates flowering time. Furthermore, NO2 suppresses hypocotyl elongation in Arabidopsis. However, information on the NO2-sensing mechanisms is scarce. The investigation focuses on NO2-mediated regulation of PIF4 protein activity and expression, suggesting that NO2 downregulates PIF4 activity to suppress hypocotyl elongation. These findings contribute to our understanding of the positive growth effects of NO2 on plants and shed light on the molecular mechanisms underlying NO2-mediated plant growth regulation.

Academic Significance and Societal Importance of the Research Achievements

本研究は、二酸化窒素センシングに関与するタンパク質遺伝子の特定とその機能の究明を目指すものであり、二酸化窒素の植物生理作用に関する新しい研究領域の創出に繋がる。また、二酸化窒素は自然にあるものであるが故に、省資源・省エネルギー、低コスト、無環境負荷の夢のような農業技術の開発が展望できる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2022 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (4 results)

  • [Journal Article] 大気中二酸化窒素による植物成長促進2022

    • Author(s)
      高橋美佐
    • Journal Title

      生物資源ゲノム解析拠点ニュースレター

      Volume: 9 Pages: 18-18

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Nitrogen Dioxide at Ambient Concentrations Induces Nitration and Degradation of PYR/PYL/RCAR Receptors to Stimulate Plant Growth: A Hypothetical Model2019

    • Author(s)
      Takahashi Misa、Morikawa Hiromichi
    • Journal Title

      Plants

      Volume: 8 Issue: 7 Pages: 198-198

    • DOI

      10.3390/plants8070198

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dual nitrogen species involved in foliar uptake of nitrogen dioxide in Arabidopsis thaliana2019

    • Author(s)
      Takahashi Misa、Arimura Gen-Ichiro、Morikawa Hiromichi
    • Journal Title

      Plant Signaling & Behavior

      Volume: 14 Issue: 4 Pages: e1582263-e1582263

    • DOI

      10.1080/15592324.2019.1582263

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrate, but not nitrite, derived from nitrogen dioxide accumulates in Arabidopsis leaves following exposure to 15N-labeled nitrogen dioxide2019

    • Author(s)
      Takahashi Misa、Morikawa Hiromichi
    • Journal Title

      Plant Signaling & Behavior

      Volume: 14 Issue: 2 Pages: 1559579-1559579

    • DOI

      10.1080/15592324.2018.1559579

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel role for PsbO1 in photosynthetic electron transport as suggested by its light-triggered selective nitration in Arabidopsis thaliana2018

    • Author(s)
      Takahashi Misa、Morikawa Hiromichi
    • Journal Title

      Plant Signaling & Behavior

      Volume: 13 Issue: 9 Pages: e1513298-e1513298

    • DOI

      10.1080/15592324.2018.1513298

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 大気二酸化窒素によるPIF4の転写活性抑制を介したシロイヌナズナの胚軸伸長抑制2022

    • Author(s)
      1. 高橋美佐、坂本 敦、森川弘道
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Atmospheric NO2 suppresses the transcriptional activity of PIF4 to suppress hypocotyl elongation in Arabidopsis2022

    • Author(s)
      Misa Takahashi, Atsushi Sakamoto and Hiromichi Morikawa
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Atmospheric nitrogen dioxide inhibits binding of PIF4 to the promoter region of auxin pathway genes to inhibit hypocotyl elongation in Arabidopsis2020

    • Author(s)
      1. 高橋美佐、坂本 敦、森川弘道
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 二酸化窒素によるシロイヌナズナ胚軸伸長抑制にはPIF4が関与している2018

    • Author(s)
      高橋美佐、坂本 敦、森川弘道
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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