Effects of neonicotinoid and its transformation products on the cranial nerve system of medaka (Oryzias latipes) embryos
| Project/Area Number |
18K05930
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 41050:Environmental agriculture-related
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| Research Institution | Suzuka National College of Technology |
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Principal Investigator |
KAI HOTAKA 鈴鹿工業高等専門学校, その他部局等, 准教授 (50518321)
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| Co-Investigator(Kenkyū-buntansha) |
山口 雅裕 鈴鹿工業高等専門学校, その他部局等, 教授 (00360660)
石橋 弘志 愛媛大学, 農学研究科, 准教授 (90403857)
石橋 康弘 熊本県立大学, 環境共生学部, 教授 (00212928)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ネオニコチノイド系農薬 / 環境変化体 / 脳神経系 / 免疫染色 / メダカ / ネオニコチノイド / ヒメダカ / 発現変動遺伝子 / 毒性影響 |
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| Outline of Final Research Achievements |
Previous studies have suggested that neonicotinoid pesticide transformation products in water environments (NN-PTPWs) show high binding potency to nicotinic acetylcholine receptors in insects and other organisms compared to the parent compound. Furthermore, several NN-PTPWs may affect the cranial nerve system via intracellular receptors in aquatic organisms such as fish. In this study, we investigated the effects of NN-PTPWs on the embryonic cranial nerve system in medaka (Oryzias latipes). The fluorescent immunostaining analysis revealed that NN-PTPW had a severe effect on the embryonic nerve system than the parent compound, and that the optic nerve is one of the most affected cranial nerves. These results suggest that NN-PTPW has an adverse effect on the cranial nerve system of medaka embryos.
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| Academic Significance and Societal Importance of the Research Achievements |
ネオニコチノイド系農薬環境変化体（NN-PTPWs）が水生生物に及ぼす影響は不明な点が多い。本研究では、NN-PTPWsを対象として、メダカ脳神経系への影響について、異なる成長段階に対する毒性影響評価、免疫染色による脳神経系観察、発現変動遺伝子解析と毒性発現機序の予測を通して、個体―組織―細胞レベルにおいて包括的に解析した。そして、NN-PTPWsによる脳神経系への作用濃度・毒性影響とその発現機序の一部を明らかにでき、生態リスク評価のための科学的根拠が得られた。
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Report
(4 results)
Research Products
(1 results)
