| Project/Area Number |
18K05936
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
福井 えみ子 宇都宮大学, 農学部, 教授 (20208341)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 繁殖 / 生殖 / 着床 / 受胎 / 妊娠 / 胚培養 / 体外受精 / 胚盤胞 |
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| Outline of Final Research Achievements |
In vitro fertilization (IVF) embryo transfer has low conception and fertility rates. Despite many previous studies, there has been no improvement. This indicates the need for a novel approach. We have been studying implantation and the establishment of pregnancy, and have reported new findings on the regulatory mechanisms of protein expression and degradation during the acquisition of implantation ability in mouse embryos. Using these molecular mechanisms as a guide, we have developed an approach to improve the implantation ability of mouse IVF-derived blastocysts by activating gene and protein expression in the blastocyst in a culture system and transferring the embryo to the uterus with an enhanced ability to respond to maternal signals.
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| Academic Significance and Societal Importance of the Research Achievements |
体外受精胚の移植では受胎率および産子率が低い。ウシ体外受精由来胚盤胞の受胎率は約40%、産子率は約20％と低い。ウシの体外受精と着床前の体外発生培養法は確立されており、体細胞クローンウシも多数作出されている今日ではあるが、何故産子生産率は低いのか？これまでの多くの研究にもかかわらず改善されていない。本研究では、胚の着床能力獲得過程に関する分子機構の解析を行なった。また、それらの分子機構を指標として、胚盤胞における遺伝子とタンパク質の発現を培養系で賦活化して子宮へ胚移植することで着床能力を改善する手法を構築した。体外受精胚の移植における低い受胎率および産子率の問題の解明と対処法の樹立に貢献する。
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