Transcriptomic signature of cumulus cells that underpin the functional completeness of bovine oocytes

• Project/Area Number: 18K05937
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Sugimura Satoshi 東京農工大学, (連合)農学研究科(研究院), 准教授 (00728454)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 卵母細胞 / 発生能 / 卵胞細胞 / ウシ / 卵子 / 成熟 / 卵丘細胞 / cAMP / PKA / 卵胞発育 / 体外成熟 / トランスクリプトーム

Outline of Final Research Achievements

The developmental competence, which is a function of oocytes, is regulated by follicular cells such as cumulus cells and granulosa cells that surround them. So far, I showed that follicle growth treatment (FGT) with FSH improves the developmental competence of oocytes. From RNA-seq and metabolome analysis, it was shown that FGT with FSH suppresses excessive glucose metabolism of follicle cells, thereby suppressing follicle cell regression and migration. Hence I demonstrated that the developmental competence of oocytes is acquired and maintained by the coupling of the follicular cell-oocyte signaling network.

Academic Significance and Societal Importance of the Research Achievements

本研究において、卵母細胞の機能的完全性、すなわち、発生能の獲得・維持機構の一旦が明らかになった。卵母細胞の発生能獲得機構の全容解明の一助になるだけでなく、本研究の知見に基づいた卵母細胞の体外培養系の開発により、家畜の効率的生産、育種改良の迅速化が期待される。

Research Products

• [Int'l Joint Research] University of New South Wales(オーストラリア)
• [Journal Article] Changes in ovarian morphology and hormone concentrations associated with reproductive seasonality in wild large Japanese field mice ( Apodemus speciosus) (2021)
  • Author(s): Komatsu K, Murata K, Iwasaki T, Tokita S, Yonekura S, Sugimura S, Fujishima Y, Nakata A, Miura T, Yamashiro H.
  • Journal Title: Anim Reprod Volume: 18 Issue: 4
  • DOI: 10.1590/1984-3143-ar2021-0067
  • Peer Reviewed / Open Access
• [Journal Article] Morphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes (2021)
  • Author(s): Suzuki Ryosuke、Okada Mai、Nagai Hiroki、Kobayashi Junichi、Sugimura Satoshi
  • Journal Title: Theriogenology Volume: 166 Pages: 55-63
  • DOI: 10.1016/j.theriogenology.2021.02.021
  • Peer Reviewed
• [Journal Article] Abnormal cleavage is involved in the self-correction of bovine preimplantation embryos (2021)
  • Author(s): Nagai H, Okada M, Nagai Y, Sakuraba Y, Okae H, Suzuki R, Sugimura S
  • Journal Title: Biochemical and Biophysical Research Communications Volume: in press
  • Peer Reviewed
• [Journal Article] A microwell culture system that allows group culture and is compatible with human single media (2018)
  • Author(s): Ieda S, Akai T, Sakaguchi Y, Shimamura S, Sugawara A, Kaneda M, Matoba S, Kagota M, Sugimura S, Kaijima H
  • Journal Title: Journal of Assisted Reproduction and Genetics Volume: 35 Issue: 10 Pages: 1869-1880
  • DOI: 10.1007/s10815-018-1252-z
  • Peer Reviewed
• [Journal Article] Live-cell imaging of nuclear-chromosomal dynamics in bovine in vitro fertilised embryos (2018)
  • Author(s): Yao T, Suzuki R, Furuta N, Suzuki Y, Kabe K, Tokoro M, Sugawara A, Yajima A, Nagasawa T, Matoba S, Yamagata K, Sugimura S.
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 7460-7460
  • DOI: 10.1038/s41598-018-25698-w
  • Peer Reviewed / Open Access
• [Journal Article] Effect of pre-<i>in vitro</i> maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle (2018)
  • Author(s): Sugimura S, Yamanouchi T, Palmerini MG, Hashiyada Y, Imai K, Gilchrist RB.
  • Journal Title: Journal of Reproduction and Development Volume: 64 Issue: 3 Pages: 233-241
  • DOI: 10.1262/jrd.2018-009
  • NAID: 130007409957
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 異常卵割のウシ胚の自己修復への関与 (2021)
  • Author(s): 長井博紀 、岡田真依 、長井陽子、桜庭喜行、岡江寛明、鈴木亮祐、杉村智史
  • Organizer: 第5回日本胚移植技術研究会
• [Presentation] ウシIVF胚盤胞におけるインプリント遺伝子のアレル特異的発現解析 (2021)
  • Author(s): 木村吉拓、金田正弘、岡江寛明、杉村智史
  • Organizer: 第114回日本繁殖生物学会
• [Presentation] ウシ体外受精胚のコンパクション時に排除される動態解析 (2020)
  • Author(s): 長井博紀、杉村智史
  • Organizer: 第113回日本繁殖生物学会大会
• [Presentation] Analysis of chromosomal abnormality of bovine in vitro fertilized embryos based on next-generation sequencing (2020)
  • Author(s): Okada M, Nagai Y, Matoba S, Sakuraba Y, Sugimura S
  • Organizer: International Embryo Technology Society 46th Annual Conference
  • Int'l Joint Research
• [Presentation] Follicular guidance for oocyte developmental competence (2018)
  • Author(s): Sugimura S, Richani D, Gilchrist RB
  • Organizer: 10th International Ruminant Reproduction Symposium
  • Int'l Joint Research / Invited