Treatment of mastitis using temporal cessation of milking

• Project/Area Number: 18K05946
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Hiroshima University
• Principal Investigator: Isobe Naoki 広島大学, 統合生命科学研究科(生), 教授 (80284230)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 乳房炎 / 乳量 / ショート乾乳 / 抗菌因子 / 搾乳頻度 / 乳牛 / ヤギ / 自然免疫 / 体細胞数 / 低頻度搾乳

Outline of Final Research Achievements

The aim of the present study was to establish the treatment method for mastitis using maximum production of antimicrobial components by changing milking frequency. The cessation of milking for 3 days decreased milk yield but increased antimicrobial components production. Then, increased frequency of milking recovered milk yield same as before cessation of milking. This method did not affect on milk yield and antimicrobial components of other udders. The cessation of milking in mastitic udders also resulted in the elevation of antimicrobial components. These results suggest that this method is appropriate for the treatment of mastitis through the function of antimicrobial components.

Academic Significance and Societal Importance of the Research Achievements

乳房炎治療のための最新技術であるショート乾乳は日本で実際に行われている技術であり，実際に成果が得られているが，治療効果のメカニズムは不明であった．本研究の成果から、ショート乾乳により乳中抗菌因子濃度を飛躍的に高めることができることが明らかになった。これらの成果から、抗生物質を用いることなく搾乳を停止するだけで行える極めて安価で簡易な方法の有効性を明らかにすることができ、本手法の有効性を提案できた．

Research Products

• [Journal Article] Association of endometritis and ovarian follicular cyst with mastitis in dairy cows (2021)
  • Author(s): Purba FY, Suzuki N, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 2 Pages: 338-343
  • DOI: 10.1292/jvms.20-0652
  • NAID: 130007995008
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in ionized calcium concentration in the blood of dairy cows with peracute coliform mastitis (2020)
  • Author(s): Hisaeda K, Koshiishi T, Sasaki A, Shinozuka Y, Isobe N and Kawai K
  • Journal Title: Journal of Veterinary Medical Science Volume: 82 Issue: 4 Pages: 457-462
  • DOI: 10.1292/jvms.19-0678
  • NAID: 130007829875
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Effects of intrauterine infusion of bacterial lipopolysaccharides on the mammary gland inflammatory response in goats. (2020)
  • Author(s): Purba FY, Ueda J, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 219: Pages: 109972-109972
  • DOI: 10.1016/j.vetimm.2019.109972
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of oral administration of colostrum whey to peripartum goat on antimicrobial peptides in postpartum milk (2020)
  • Author(s): Isobe N, Matsukawa S, Kubo K, Ueno K, Sugino T, Nii T, Yoshimura Y
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13365
  • Peer Reviewed
• [Journal Article] Seasonal variations in the concentration of antimicrobial components in milk of dairy cows. (2020)
  • Author(s): Suzuki N, Purba FY, Hayashi Y, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13427
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Outcome prediction from the first examination in clinical mastitis using ultrasonography in dairy cows. (2020)
  • Author(s): Suzuki N, Kurose T, Kaneko S, Haraguchi A, Isobe N
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13452
  • Peer Reviewed
• [Journal Article] Translocation of intrauterine-infused bacterial lipopolysaccharides to the mammary gland in dexamethasone-treated goats. (2020)
  • Author(s): Purba FY, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Reproduction in Domestic Animals Volume: 55: Issue: 12 Pages: 1688-1697
  • DOI: 10.1111/rda.13820
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Immune response during the onset of coliform mastitis in dairy cows vaccinated with STARTVAC (2020)
  • Author(s): Kazuhiro Kawai, Yasuha Kondo, Yasunori Shinozuka, Ryusaku Kawata, Sohei Kaneko, Hidetomo Iwano, Masafumi Enokidani, Aiko Watanabe, Fika Yuliza-Purba, Isobe N and Tomomi Kurumisawa
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13502
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Production of antimicrobial peptide S100A8 in the goat mammary gland and effect of intra-mammary infusion of lipopolysaccharide on S100A8 concentration in milk. (2019)
  • Author(s): Purba FY, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Journal of Dairy Science Volume: 102 Issue: 5 Pages: 4674-4681
  • DOI: 10.3168/jds.2018-15396
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide (2018)
  • Author(s): Shinozuka Y, Kawai K, Sato R, Higashitani A, Hamamoto Y, Okita M, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 2 Pages: 242-246
  • DOI: 10.1292/jvms.17-0615
  • NAID: 130006339635
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Effects of colostrum whey on immune function in the digestive tract of goats (2018)
  • Author(s): Matsukawa Shoko、Ueno Kazutoshi、Sugino Toshihisa、Yoshimura Yukinori、Isobe Naoki
  • Journal Title: Animal Science Journal Volume: 89 Issue: 8 Pages: 1152-1160
  • DOI: 10.1111/asj.13027
  • Peer Reviewed
• [Journal Article] Effect of intramammary lipopolysaccharide infusion on milk pH of uninfused udder in goat (2018)
  • Author(s): Shinozuka Y, Kawai K, Sato R, Higashitani A, Ueno D, Okita M, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 8 Pages: 1287-1290
  • DOI: 10.1292/jvms.18-0280
  • NAID: 130007437812
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Investigation of the binding of goat cathelicidin-7 to lipopolysaccharide and leucocidal suppression of pro-inflammatory cytokines. (2018)
  • Author(s): Nishikawa M, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Small Ruminant Research Volume: 168 Pages: 101-106
  • DOI: 10.1016/j.smallrumres.2018.10.006
  • Peer Reviewed
• [Presentation] 頻回搾乳がヤギ乳汁中抗菌因子濃度に及ぼす影響． (2021)
  • Author(s): 原田梨花、新居隆浩、吉村幸則、磯部直樹：
  • Organizer: 日本畜産学会第128回大会
• [Presentation] ヤギ乳房へのメントール塗布が抗菌因子産生に及ぼす影響． (2021)
  • Author(s): 大野瑠璃、新居隆浩、吉村幸則、磯部直樹：
  • Organizer: 日本畜産学会第128回大会
• [Presentation] Lipopolysaccharides infusion into the uterus causes inflammation in the mammary gland． (2021)
  • Author(s): Fika Yuliza Purba1, Takahiro Nii, Yukinori Yoshimura, Naoki Isobe
  • Organizer: 日本畜産学会第128回大会
• [Presentation] Inflammatory responses in the mammary gland after intrauterine infusion of lipopolysaccharide in dexamethasone-treated goats． (2020)
  • Author(s): Purba FY,Nii T, Yoshimura Y, Isobe N
  • Organizer: 1st International Veterinay Science Virtual Conference (ICVS)
  • Int'l Joint Research
• [Presentation] Antimicrobial components for the local defense in ruminant mammary gland. (2020)
  • Author(s): Isobe N:
  • Organizer: The 3rd International Conference of Animal Science and Technology (ICAST 3)
  • Int'l Joint Research / Invited
• [Presentation] 他の臓器から移行するLPSによる乳房炎の可能性 (2019)
  • Author(s): 磯部直樹
  • Organizer: 中四国乳房炎協議会第29回勉強会
  • Invited
• [Presentation] 乳房炎の意外な原因 (2019)
  • Author(s): 磯部直樹
  • Organizer: 第43回NOSAI診療技術研究発表会
  • Invited
• [Presentation] 低頻度搾乳が乳汁中抗菌因子濃度に及ぼす影響. (2019)
  • Author(s): 原田梨花、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 第6回乳房炎サマーキャンプ
• [Presentation] ヤギにおけるStaphylococcus aureus刺激による乳房炎に ショート乾乳を実施した時の乳中抗菌因子濃度変化 (2019)
  • Author(s): 石本佳久、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 第6回乳房炎サマーキャンプ
• [Presentation] Effect of oral administration of colostrum on the antimicrobial peptides concentration in goat milk. (2019)
  • Author(s): Isobe N, Kubo K, Ueno K, Nii T, Yoshimura Y
  • Organizer: The 8th International Seminar on Tropical Animal Production (ISTAP)
  • Int'l Joint Research
• [Presentation] Effect of low milking frequency on the concentration of antimicrobial peptides in goat milk . (2019)
  • Author(s): Harada R, Nii T, Yoshimura Y, Isobe N
  • Organizer: The 8th International Seminar on Tropical Animal Production (ISTAP)
  • Int'l Joint Research
• [Presentation] Antimicrobial peptides in mammary glnad. (2019)
  • Author(s): Isobe N
  • Organizer: Seminar of Animal Diseases and Food Safety
  • Int'l Joint Research / Invited
• [Presentation] 乳汁中抗菌因子の季節変化と甲状腺機能の関係. (2019)
  • Author(s): 鈴木直樹・Fika Yuliza Purba・原田梨花・石場優希・新居隆浩・吉村幸則・磯部直樹：
  • Organizer: 第24回日本乳房炎研究会学術集会
• [Presentation] 低頻度搾乳が乳汁中抗菌因子濃度に及ぼす影響. (2019)
  • Author(s): 原田梨花、新居隆浩、吉村幸則、磯部直樹：
  • Organizer: 第24回日本乳房炎研究会学術集会
• [Presentation] Staphylococcus aureus刺激による乳房炎に ショート乾乳を実施した時の乳中抗菌因子濃度変化 (2019)
  • Author(s): 石本佳久、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 第24回日本乳房炎研究会学術集会
• [Presentation] 乳房炎の意外な原因 (2019)
  • Author(s): 磯部直樹
  • Organizer: 第5回広島大学酪農技術セミナー
  • Invited
• [Presentation] Effect of tentative cessation of milking for 3 days on innate immune components in goat milk. (2018)
  • Author(s): Naoki Isobe, Jo Ueda, Yukinori Yoshimura
  • Organizer: 2018 Annual Meeting of the American Dairy Science Association
  • Int'l Joint Research
• [Presentation] Effect of mastitis on the reproductive functions in pregnant goat (2018)
  • Author(s): Isobe N, Kodama N, Nii T, Yoshimura Y
  • Organizer: 18th Asian Australasian Animal Production Congress
  • Int'l Joint Research
• [Presentation] Effect of Estradiol on Antimicrobial Components in Milk of Goat Treated with Temporal Cessation of Milking. (2018)
  • Author(s): Ishimoto Y, Nii T, Yoshimura Y, Isobe N
  • Organizer: 18th Asian Australasian Animal Production Congress
  • Int'l Joint Research
• [Presentation] Changes in Inflammatory Components of the Mammary Gland after Intrauterine Infusion of Lipopolysaccharide in Goats (2018)
  • Author(s): Purba, F. Y., Nii T, Yoshimura, Y. Isobe, N
  • Organizer: 18th Asian Australasian Animal Production Congress
  • Int'l Joint Research
• [Presentation] Different concentration of antimicrobial components in bovine milk infected with different pathogens. (2018)
  • Author(s): Isobe N, Kawai K, Shinozuka Y, Purba YF, Nii T, Yoshimura Y：
  • Organizer: The 30th World Buiatrics Congress .
  • Int'l Joint Research
• [Presentation] 周産期ヤギへの初乳の経口投与が乳汁中抗菌因子濃度に及ぼす影響 (2018)
  • Author(s): 久保海太・上野和俊・新居隆浩・吉村幸則・磯部直樹
  • Organizer: 平成30年度第68回関西畜産学会大会
• [Presentation] ショート乾乳にEstradiolを併用した時の乳中抗菌因子濃度変化 (2018)
  • Author(s): 石本佳久、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 第２３回 日本乳房炎研究会学術集会
• [Presentation] Changes in inflammatory components of mammary gland after intrauterine infusion of lipopolysaccharide in goats (2018)
  • Author(s): Fika Purba、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 日本畜産学会第125回大会
• [Presentation] 低頻度搾乳が乳汁中抗菌因子濃度に及ぼす影響 (2018)
  • Author(s): 原田梨花、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 日本畜産学会第125回大会
• [Presentation] ヤギ片側分房の一時的な搾乳停止が乳中抗菌因子濃度に及ぼす影響 (2018)
  • Author(s): 石本佳久、新居隆浩、吉村幸則、磯部直樹
  • Organizer: 日本畜産学会第125回大会
• [Presentation] 暑熱時における乳房免疫の機能変化 (2018)
  • Author(s): 磯部直樹
  • Organizer: らくのうマザーズ、暑熱対策セミナー
  • Invited
• [Presentation] 発情すると乳房炎になる？ ー自然免疫の視点から再考するー (2018)
  • Author(s): 磯部直樹
  • Organizer: 第111回日本繁殖生物学会大会
  • Invited
• [Presentation] 周産期に初乳製剤を経口投与した時の 乳中抗菌因子濃度変化 (2018)
  • Author(s): 磯部直樹
  • Organizer: 第42回NOSAI診療技術研究発表会
  • Invited
• [Presentation] 抗菌因子に配慮した乳汁管理 (2018)
  • Author(s): 磯部直樹
  • Organizer: 東北乳房炎協議会
  • Invited
• [Remarks] 広島大学家畜生体機構学研究室ホームページ
  • URL: https://anat.hiroshima-u.ac.jp/
• [Remarks] https://home.hiroshima-u.ac.jp/anat
• [Remarks] 広島大学家畜生体機構学研究室
  • URL: https://home.hiroshima-u.ac.jp/anat/