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Is carnosine involved in skeletal muscle energy metabolism as a beta alanine pool?

Research Project

Project/Area Number 18K05955
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Egusa Ai  日本獣医生命科学大学, 応用生命科学部, 講師 (90521972)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsカルノシン / 遺伝子改変マウス / エネルギー代謝 / 運動 / ノックアウトマウス / 骨格筋 / 細胞 / イミダソールジペプチド
Outline of Final Research Achievements

The purpose of this study was to clarify the physiological role of carnosine, which exists specifically in skeletal muscle, cerebrum and olfactory bulb, in terms of energy metabolism. Carnosine is composed of β-alanine and histidine and is present in human skeletal muscle at a high concentration of 20 mM. However, its physiological effects have not been fully elucidated. In this study, we examined the effect of carnosine synthetase gene on energy metabolism using two kinds of evaluation systems: one is an evaluation system in which carnosine synthetase gene is introduced into skeletal muscle cells, and the other is an evaluation system in which the gene is deleted in mice. Carnosine was expected to be involved in the reaction of pyruvate to acetyl-CoA, resulting in increased energy production, however no direct involvement was found in ATP synthesis in skeletal muscle.

Academic Significance and Societal Importance of the Research Achievements

カルノシンは豚や鶏などの脊椎動物の骨格筋中に多く含まれており、近年では抗疲労効果や認知症予防に役立つ食品成分として機能性表示食品などとして利用されている。一方で組織中のカルノシン量を規定する因子については不明な点が多く、動物種あるいは筋線維の違いによりカルノシン量が異なる理由については明らかにされていない。骨格筋におけるカルノシンの生合成やエネルギー代謝における役割を明らかにすることで、生体内における生理的意義を明確にし、カルノシンおよびその類縁体を含む食品を、高齢化率28%を超える日本人の健康を食から支える切り札として活用したい。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Dietary GABA induces endogenous synthesis of a novel imidazole peptide homocarnosine in mouse skeletal muscles2020

    • Author(s)
      Kumrungsee Thanutchaporn、Arima Takeshi、Sato Kanako、Komaru Takumi、Sato Mikako、Oishi Yasuyuki、Egusa Ai、Yanaka Noriyuki
    • Journal Title

      Amino Acids

      Volume: 52 Issue: 5 Pages: 743-753

    • DOI

      10.1007/s00726-020-02848-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dietary GABA Induces Endogenous Synthesis of a Novel Imidazole Peptide Homocarnosine in Mouse Skeletal Muscles2020

    • Author(s)
      Thanutchaporn Kumrungsee, Takeshi Arima, Kanako Sato, Takumi Komaru, Mikako Sato , Yasuyuki Oishi , Ai Egusa, Noriyuki Yanaka
    • Journal Title

      Anino Acids

      Volume: -

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 低酸素ストレスが骨格筋細胞のカルノシン合成に及ぼす影響2021

    • Author(s)
      野口こと葉,鈴木彩夏,中尾暢宏,戸塚護,江草(雜賀)愛
    • Organizer
      第62回日本食肉研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 骨格筋細胞の由来の違いや分化誘導がカルノシン合成に及ぼす影響2021

    • Author(s)
      高野実織、鈴木彩夏、中尾暢宏、戸塚護、江草(雜賀)愛
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イミダゾールジペプチドが骨格筋代謝におよぼす影響2020

    • Author(s)
      Wu Jiawei, 江草 愛, 塩谷茂信, 佐藤謙一郎, 柳内延也, 戸塚護, 西村敏英
    • Organizer
      第74回日本栄養・食糧学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒスチジン欠乏食を給餌したカルノシン合成酵素遺伝子欠損マウスのメタボローム解析2020

    • Author(s)
      川中暢子, Wu Jiawei, 江草愛, 塩谷茂信, 佐藤謙一郎, 柳内延也, 西村敏英
    • Organizer
      第74回日本栄養・食糧学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒスチジン欠乏食を給餌したカルノシン合成酵素遺伝子欠損マウスのメタボローム解析2020

    • Author(s)
      川内暢子、WU JIAWEI、江草 愛、塩谷 茂信、佐藤 謙一郎、柳内 延也、戸塚 護、西村 敏英
    • Organizer
      日本栄養・食糧学会
    • Related Report
      2019 Research-status Report
  • [Presentation] イミダゾールジペプチドが骨格筋代謝におよぼす影響2020

    • Author(s)
      Wu Jiawei, 江草 愛、塩谷 茂信、佐藤 謙一郎、柳内 延也、戸塚 護、西村 敏英
    • Organizer
      日本栄養・食糧学会
    • Related Report
      2019 Research-status Report
  • [Presentation] hysical characteristics of carnosine synthase gene-deficient mice2019

    • Author(s)
      Ai Egusa, Nobuhiro Nakao, Takaki Saito, Nobuya Yanai, Shigenobu Shiotani, Kenichiro Sato, Mamoru Totsuka, Toshihide Nishimura
    • Organizer
      International conference on Food Factors
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] カルノシン合成酵素遺伝子ノックアウトマウスの生理機能2019

    • Author(s)
      江草 愛
    • Organizer
      日本栄養・食糧学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] カルノシン合成酵素遺伝子ノックマウスへのアンセリン経口投与によるイミダゾールジぺプチドの組織分布2019

    • Author(s)
      坂野太研、江草 愛、塩谷茂信、仲西宏樹、柳内延也、戸塚護、西村敏英
    • Organizer
      日本栄養・食糧学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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