Investigation of microRNA functions in metabolic imprinting of Japanese Black cattle

• Project/Area Number: 18K05960
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Muroya Susumu 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主席研究員 (50355062)
• Co-Investigator(Kenkyū-buntansha): 後藤 貴文 鹿児島大学, 農水産獣医学域農学系, 教授 (70294907)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ウシ / 代謝プログラミング / 胎仔 / 骨格筋 / メタボローム / トランスクリプトーム / IUGR / microRNA / 黒毛和種牛

Outline of Final Research Achievements

The pregnant cows were fed on low-nutrition (LN) or high-nutrition (HN) of their overall nutritional requirement during gestation. The longissimus thoracis muscle of Japanese Black fetal calves was analyzed by metabolomics and transcriptomics. Maternal undernutrition decreased the bodyweight and muscle weight of the fetus. The levels of amino acids (AAs) and arginine-related metabolites including glutamine were higher in the LN group than those in the HN group. Metabolite set enrichment analysis revealed that the highly different metabolites were associated with the metabolic pathways of pyrimidine, glutathione, and AAs, suggesting that MUN resulted in AA accumulation rather than protein accumulation. The expression of genes associated with energy metabolism, glycolysis, angiogenesis, and insulin signaling pathways were lower in the LN group than in the HN group.

Academic Significance and Societal Importance of the Research Achievements

母体の低栄養状態は胎仔骨格筋の発生を抑制するが、そのメカニズムや代謝に及ぼす影響についてはほとんどわかっていない。本研究では、黒毛和種妊娠牛の栄養制限が胎仔に及ぼす影響を解明することを目的とし、胎仔骨格筋の代謝物や遺伝子発現の変動の解析した。得られた成果は、黒毛和種牛の飼養方法と胎仔成長との関係についての理解に寄与し、牛の飼養管理学、生理学、さらにヒトを含む哺乳類の子宮内発育遅滞の理解に貢献するものである。

Research Products

• [Journal Article] Maternal Nutrient Restriction Disrupts Gene Expression and Metabolites Associated with Urea Cycle, Steroid Synthesis, Glucose Homeostasis, and Glucuronidation in Fetal Calf Liver (2022)
  • Author(s): Muroya Susumu、Zhang Yi、Otomaru Kounosuke、Oshima Kazunaga、Oshima Ichiro、Sano Mitsue、Roh Sanggun、Ojima Koichi、Gotoh Takafumi
  • Journal Title: Metabolites Volume: 12 Issue: 3 Pages: 203-203
  • DOI: 10.3390/metabo12030203
  • Peer Reviewed / Open Access
• [Journal Article] Maternal Nutrition During Gestation Alters Histochemical Properties, and mRNA and microRNA Expression in Adipose Tissue of Wagyu Fetuses (2022)
  • Author(s): Zhang Yi、Otomaru Konosuke、Oshima Kazunaga、Goto Yuji、Oshima Ichiro、Muroya Susumu、Sano Mitsue、Roh Sanggun、Gotoh Takafumi
  • Journal Title: Frontiers in Endocrinology Volume: 12 Pages: 797680-797680
  • DOI: 10.3389/fendo.2021.797680
  • Peer Reviewed / Open Access
• [Journal Article] Maternal Undernutrition during Pregnancy Alters Amino Acid Metabolism and Gene Expression Associated with Energy Metabolism and Angiogenesis in Fetal Calf Muscle (2021)
  • Author(s): Muroya Susumu、Zhang Yi、Kinoshita Aoi、Otomaru Kounosuke、Oshima Kazunaga、Gotoh Yuji、Oshima Ichiro、Sano Mitsue、Roh Sanggun、Oe Mika、Ojima Koichi、Gotoh Takafumi
  • Journal Title: Metabolites Volume: 11 Issue: 9 Pages: 582-582
  • DOI: 10.3390/metabo11090582
  • Peer Reviewed / Open Access
• [Journal Article] Effects of low and high levels of maternal nutrition consumed for the entirety of gestation on the development of muscle, adipose tissue, bone, and the organs of Wagyu cattle fetuses (2021)
  • Author(s): Zhang Yi、Otomaru Kounosuke、Oshima Kazunaga、Goto Yuji、Oshima Ichiro、Muroya Susumu、Sano Mitsue、Saneshima Rena、Nagao Yukiko、Kinoshita Aoi、Okamura Yasuko、Roh Sanggun、Ohtsuka Akira、Gotoh Takafumi
  • Journal Title: Animal Science Journal Volume: 92 Issue: 1
  • DOI: 10.1111/asj.13600
  • Peer Reviewed / Open Access
• [Journal Article] Difference in potential DNA methylation impact on gene expression between fast- and slow-type myofibers (2021)
  • Author(s): Oe Mika、Ojima Koichi、Muroya Susumu
  • Journal Title: Physiological Genomics Volume: 53 Issue: 2 Pages: 69-83
  • DOI: 10.1152/physiolgenomics.00099.2020
  • Peer Reviewed
• [Journal Article] MEATabolomics: Muscle and Meat Metabolomics in Domestic Animals (2020)
  • Author(s): Muroya Susumu、Ueda Shuji、Komatsu Tomohiko、Miyakawa Takuya、Ertbjerg Per
  • Journal Title: Metabolites Volume: 10 Issue: 5 Pages: 188-188
  • DOI: 10.3390/metabo10050188
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coordinate alteration of mRNA-microRNA transcriptomes associated with exosomes and fatty acid metabolism in adipose tissue and skeletal muscle in grazing cattle (2019)
  • Author(s): Muroya Susumu、Ogasawara Hideki、Nohara Kana、Oe Mika、Ojima Koichi、Hojito Masayuki
  • Journal Title: Asian-Australasian Journal of Animal Sciences Volume: - Issue: 11 Pages: 1824-1836
  • DOI: 10.5713/ajas.19.0682
  • Peer Reviewed / Open Access
• [Journal Article] Comparing pig breeds with genetically low and high backfat thickness: differences in expression of adiponectin, its receptor, and blood metabolites. (2019)
  • Author(s): Nakajima I, Kojima M, Oe M, Ojima K, Muroya S, Chikuni K
  • Journal Title: Domestic animal endocrinology Volume: 68 Pages: 54-63
  • Peer Reviewed
• [Journal Article] Metabolomic approach to key metabolites characterizing postmortem aged loin muscle of Japanese Black (Wagyu) cattle (2019)
  • Author(s): Muroya Susumu、Oe Mika、Ojima Koichi、Watanabe Akira
  • Journal Title: Asian-Australasian Journal of Animal Sciences Volume: 印刷中 Issue: 8 Pages: 1172-1185
  • DOI: 10.5713/ajas.18.0648
  • Peer Reviewed / Open Access
• [Journal Article] PSVII-25 Grazing-induced transcriptomic changes in bovine biceps femoris muscle, subcutaneous fat, and liver mRNAs and plasma exosome microRNAs. (2018)
  • Author(s): Muroya S、Ogasawara H、Nohara K、Oe M、Ojima K、Hojito M
  • Journal Title: Journal of Animal Science Volume: 96 Issue: suppl_3 Pages: 356-356
  • DOI: 10.1093/jas/sky404.783
  • Peer Reviewed
• [Presentation] 10年先にあってほしい牛肉とその研究開発を考える (2022)
  • Author(s): 室谷進
  • Organizer: 日本産肉研究会第29回学術集会
  • Invited
• [Presentation] 母牛の低栄養状態における胎仔胸最長筋のメタボローム解析 (2021)
  • Author(s): 室谷進、Y. Zhang、木下葵衣、乙丸孝之介、大島一修、後藤裕司、大島一郎、佐野光枝、盧尚健、大江美香、尾嶋孝一、後藤貴文
  • Organizer: 日本畜産学会第129回大会
• [Presentation] 子牛の強化哺乳と育成期の配合飼料給与が肥育後の肝臓の代謝調節にもたらす変化 (2021)
  • Author(s): 室谷 進、斉藤昭、大江 美香、尾嶋 孝一、後藤貴文
  • Organizer: 第128回日本畜産学会大会
• [Presentation] Effect of poor maternal nutrition on skeletal muscle microRNA expression of foetal calves (2020)
  • Author(s): S. Muroya, Y. Zhang, A. Kinoshita, K. Oshima, Y. Gotoh, I. Oshima, K. Otomaru, M. Sano, S. Roh, M. Futohashi, M. Oe, K. Ojima, T. Gotoh
  • Organizer: Book of Abstracts of the 71st Annual Meeting of the European Federation of Animal Science
  • Int'l Joint Research
• [Presentation] Metabolomic changes and the relevant pathways in longissimus muscle of Japanese Black cattle during postmortem aging (2019)
  • Author(s): S. Muroya, M. Oe, K. Ojima, A. Watanabe
  • Organizer: ICoMST 2019 (65th International Congress of Meat Science and Technology)
  • Int'l Joint Research
• [Presentation] Impact of a high nutrient milk replacer feeding followed by concentrate feeding in rearing period on the liver DNA methylation in adult cattle (2019)
  • Author(s): S. Muroya, T. Abe, K. Saito, A. Saito, M. Oe, K. Ojima, T. Gotoh
  • Organizer: DOHaD 2019 (11th DOHaD World Congress)
  • Int'l Joint Research
• [Presentation] 筋線維型間でDNAメチル化率の異なる遺伝子群の遺伝子オントロジー解析 (2019)
  • Author(s): 大江美香, 尾嶋孝一, 室谷進
  • Organizer: 日本畜産学会第126回大会
• [Presentation] Effects of maternal nutrition on fetal development in Wagyu cows (2019)
  • Author(s): Zhang Yi, 實島伶奈, 長尾有希子, 木下葵衣, 大島一修, 後藤 裕司, 大島一郎 , 佐野光枝, 室谷 進, 盧尚建, 太箸誠, 岡村保子, 後藤貴文
  • Organizer: 日本畜産学会第125回大会
• [Presentation] Metabolome analysis of porcine fast and slow type muscles during postmortem aging (2018)
  • Author(s): M. Oe, K. Ojima, S. Muroya
  • Organizer: 2018 ASAS-CSAS Annual Meeting and Trade Show
  • Int'l Joint Research