Study for interspecies transmission of animal viruses using metagenomics approach

• Project/Area Number: 18K05977
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Azabu University
• Principal Investigator: Nagai Makoto 麻布大学, 獣医学部, 教授 (10540669)
• Co-Investigator(Kenkyū-buntansha): 大井 徹 石川県立大学, 生物資源環境学部, 教授 (10201964)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 家畜 / 野生動物 / ウイルス検出 / 種間伝播（スピルオーバー） / メタゲノム解析 / 新規ウイルス / 遺伝子変異 / イノシシ / 豚 / 糞便ウイルス / 種間伝播 / ウイルス遺伝子多様性 / ウイルス遺伝子組換え / 疫学的調査 / 網羅的解析 / 遺伝子解析 / 動物ウイルス / 糞便

Outline of Final Research Achievements

Using metagenomics approach, novel viruses and viruses suggesting past interspecies transmission were identified from livestock and wild animals and virologically analyzed. An orthoreovirus firstly isolated from wild boar was thought to be a reassortant virus generated through interspecies transmission between human, lion at a zoo in Japan, and bat. We also identified sapoviruses and enteroviruses carrying PL-CP from wild boar for the first time. Furthermore, six viruses including sapelovirus, posavirus, sapovirus, torovirus, enterovirus G, and pestivirus, which show unique genetic property, were discovered from Japanese pigs.

Academic Significance and Societal Importance of the Research Achievements

ヒトの新興感染症の多くは野生動物からの動物由来感染症であることがわかってきた。家畜においては野生動物から感染するリスク以外に、家畜から野生動物に感染症が伝播する可能性も指摘されている。このような状況下では家畜と野生動物の間に種間伝播（スピルオーバー）が起こり、新しいウイルスが生じる可能性がある。家畜と野生動物が保有するウイルスを検出し解析することは、種間伝播するウイルスを監視するばかりではなく、ウイルスの進化を考察する上でも重要である。

Research Products

• [Journal Article] Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan (2021)
  • Author(s): Kasahara‐Kamiie Miwako、Kagawa Mitsuo、Shiokawa Mai、Sunaga Fujiko、Fukase Yuka、Aihara Naoyuki、Shiga Takanori、Kamiie Junichi、Aoki Hiroshi、Nagai Makoto
  • Journal Title: Transboundary and Emerging Diseases Volume: 印刷中 Issue: 4 Pages: 1-9
  • DOI: 10.1111/tbed.14149
  • Peer Reviewed
• [Journal Article] Isolation and characterization of mammalian orthoreovirus type 3 from a fecal sample from a wild boar in Japan (2021)
  • Author(s): Zhang Wenjing、Kataoka Michiyo、Doan Yen Hai、Oi Toru、Furuya Tetsuya、Oba Mami、Mizutani Tetsuya、Oka Tomoichiro、Li Tian-Cheng、Nagai Makoto
  • Journal Title: Archives of Virology Volume: 印刷中 Issue: 6 Pages: 1671-1680
  • DOI: 10.1007/s00705-021-05053-7
  • Peer Reviewed
• [Journal Article] Multiple genotypes of enterovirus G carrying a papain-like cysteine protease (PL-CP) sequence circulating on two pig farms in Japan: first identification of enterovirus G10 carrying a PL-CP sequence (2020)
  • Author(s): Sekiguchi Yuya、Nagata Ayaka、Sunaga Fujiko、Oi Toru、Imai Ryo、Madarame Hiroo、Katayama Yukie、Oba Mami、Okabayashi Tamaki、Misawa Naoaki、Oka Tomoichiro、Mizutani Tetsuya、Nagai Makoto
  • Journal Title: Archives of Virology Volume: 165 Issue: 12 Pages: 2909-2914
  • DOI: 10.1007/s00705-020-04816-y
  • Peer Reviewed
• [Journal Article] Genetic diversity of enterovirus G detected in faecal samples of wild boars in Japan: identification of novel genotypes carrying a papain-like cysteine protease sequence (2020)
  • Author(s): Nagata Ayaka、Sekiguchi Yuya、Oi Toru、Sunaga Fujiko、Madarame Hiroo、Imai Ryo、Sano Kaori、Katayama Yukie、Omatsu Tsutomu、Oba Mami、Furuya Tetsuya、Shirai Junsuke、Okabayashi Tamaki、Misawa Naoaki、Oka Tomoichiro、Mizutani Tetsuya、Nagai Makoto
  • Journal Title: Journal of General Virology Volume: 101 Issue: 8 Pages: 840-852
  • DOI: 10.1099/jgv.0.001446
• [Journal Article] Complete genome sequencing and genetic analysis of a Japanese porcine torovirus strain detected in swine feces. (2020)
  • Author(s): Fujii Yuki、Kashima Yuki、Sunaga Fujiko、Aoki Hiroshi、Imai Ryo、Sano Kaori、Katayama Yukie、Omatsu Tsutomu、Oba Mami、Furuya Tetsuya、Tsuzuku Satoko、Ouchi Yoshinao、Shirai Junsuke、Mizutani Tetsuya、Oka Tomoichiro、Nagai Makoto
  • Journal Title: Archives of virology Volume: 165 Issue: 2 Pages: 471-477
  • DOI: 10.1007/s00705-019-04514-4
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates. (2019)
  • Author(s): Aoki Hiroshi、Sunaga Fujiko、 Ochiai Hideharu、Masuda Tsuneyuki、Ito Mika、Akagami Masataka、Naoi Yuki、Sano Kaori、Katayama Yukie、Omatsu Tsutomu、Oba Mami、Sakaguchi Shoichi、Furuya Tetsuya、Ouchi Yoshinao、Shirai Junsuke、Mizitani Tetsuya、Oka Tomoichiro、Nagai Makoto
  • Journal Title: Archives of virology Volume: 164 Issue: 8 Pages: 2147-2151
  • DOI: 10.1007/s00705-019-04289-8
  • Peer Reviewed
• [Journal Article] Complete genome sequencing and genetic characterization of porcine sapovirus genogroup (G) X and GXI: GVI, GVII, GX, and GXI sapoviruses share common genomic features and form a unique porcine SaV clade (2019)
  • Author(s): Sunaga Fujiko、Masuda Tsuneyuki、Aoki Hiroshi、Ito Mika、Sano Kaori、Naoi Yuki、Katayama Yukie、Omatsu Tsutomu、Oba Mami、Furuya Tetsuya、Shirai Junsuke、Mizutani Tetsuya、Oka Tomoichiro、Nagai Makoto
  • Journal Title: Infection, Genetics and Evolution Volume: 75 Pages: 103959-103959
  • DOI: 10.1016/j.meegid.2019.103959
  • Peer Reviewed
• [Journal Article] First identification of Sapoviruses in wild boar. (2019)
  • Author(s): Katsuta Risako、Sunaga Fujiko、Oi Toru、Doan Yen Hai、Tsuzuku Satoko、Suzuki Yoshihisa、Sano Kaori、Katayama Yukie、Omatsu Tsutomu、Oba Mami、Furuya Tetsuya、Ouchi Yoshinao、Shirai Junsuke、Mizutani Tetsuya、Oka Tomoichiro、Nagai Makoto
  • Journal Title: Virus Research Volume: 271 Pages: 197680-197680
  • DOI: 10.1016/j.virusres.2019.197680
  • Peer Reviewed
• [Journal Article] Complete genomic analysis and molecular characterization of Japanese porcine sapeloviruses (2019)
  • Author(s): Sunaga F, Masuda T, Ito M, Akagami M, Naoi Y, Sano K, Katayama Y, Omatsu T, Oba M, Sakaguchi S, Furuya T, Yamasato H, Ouchi Y, Shirai J, Mizutani T, Nagai M.
  • Journal Title: Virus Genes Volume: 55 Issue: 2 Pages: 198-208
  • DOI: 10.1007/s11262-019-01640-8
  • Peer Reviewed
• [Journal Article] Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan (2018)
  • Author(s): Masuda T, Sunaga F, Naoi Y, Ito M, Takagi H, Katayama Y, Omatsu T, Oba M, Sakaguchi S, Furuya T, Yamasato H, Shirai J, Makino S, Mizutani T, Nagai M
  • Journal Title: Virus research. Volume: 257 Pages: 68-73
  • DOI: 10.1016/j.virusres.2018.09.003
  • Peer Reviewed / Int'l Joint Research