| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In the present study, we investigated on the microRNA (miRNA) contained in bovine endometrial cells and trophoblasts-derived exosomes, and attempted to elucidate the molecular mechanism of cell function by miRNA. As a result, some specific miRNAs from the endometrial cells and trophoblasts may bind to the 3'-untranslated and translated regions of pregnancy-related glycoprotein (PAG) and placenta lactogen (CSH2) mRNA. Inverse correlation of the expression kinetics was observed between trophoblast-specific mRNA and specific miRNA, suggesting that specific miRNA may regulate the expression of PAG and placenta lactogen protein. These results suggest that miRNA contributes to the interaction between endometrial cells and trophoblasts in implantation and placentation in cattle.
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