Biogeography of Kudoa spp. (Myxosporea: Multivalvulida) distributed in Asian Seas with special reference to risk management of Kudoa food poisoning

• Project/Area Number: 18K05995
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Yamaguchi University
• Principal Investigator: Sato Hiroshi 山口大学, 共同獣医学部, 教授 (90211945)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: クドア食中毒 / 多殻目粘液胞子虫 / 海産魚 / 種多様性 / Kudoa / Unicapsula / rDNA / 生物地理学 / クドア / 粘液胞子虫 / 南シナ海 / リスク評価

Outline of Final Research Achievements

After identification of Kudoa septempunctata as the cause of outbreaks of new food poisoning after consumption of fresh olive flounder, named 'Kudoa food poisoning', K. hexapunctata in the Thunnus tunas, K. iwatai in the sea bass, and Unicapsula seriolae in the greater amberjack have been added as potential causes of Kudoa food poisoning. However, identification of multivalvulid species of the genera Kudoa and Unicapsula is hampered by the paucity of taxonomic and biogeographical knowledgement of the multivalvulids. This study collected a variety of Kudoa spp. and Unicapsula spp. from commercial marine fishes in the waters around Japan and China, and characterized individually their morphological and molecular-genetic data as taxonomic units. Accumulation of rDNA nucleotide sequences of variable multivalvulid species with taxonomic reliability enables feasible identification of the cause of Kudoa food poisoning.

Academic Significance and Societal Importance of the Research Achievements

本研究では日本近海、ベーリング海、南シナ海、ジャワ海、大西洋に生息する市販海産魚に寄生する多殻目粘液胞子虫について調べ、既知種13種、新種11種を検出するとともに、胞子の形態学的観察とrDNAならびにmtDNA塩基配列情報を集積した。クドア食中毒の原因となり、また、養殖現場で重要な寄生虫病となる多殻目粘液胞子の種多様性の把握とDNA情報の集積は、食品衛生あるいは養殖現場、検疫現場での種同定において重要な基盤情報となる。本研究で得られた成果は、論文あるいはDNA公開リポジトリDDBJ等で公開している。

Research Products

• [Int'l Joint Research] Guangdong Ocean University/Qingdao Agricultural University/Chinese Academy of Sciences(中国)
• [Int'l Joint Research] Airlangga University/Tanjungpura University(インドネシア)
• [Int'l Joint Research] University of Kalyani(インド)
• [Int'l Joint Research] Cooperative Oxford Laboratory(米国)
• [Int'l Joint Research] Russian Academy of Sciences(ロシア連邦)
• [Int'l Joint Research] 広東海洋大学/中国科学院/Zhejiang University(中国)
• [Int'l Joint Research] アイルランガ大学/ガジャマダ大学/Hasanuddin University(インドネシア)
• [Int'l Joint Research] University of the Philippines Manila(フィリピン)
• [Int'l Joint Research] 中国科学院水生生物研究所/広東海洋大学(中国)
• [Int'l Joint Research] サッサリ大学(イタリア)
• [Int'l Joint Research] エジンバラ大学(英国)
• [Journal Article] First report of three multivalvulid species (Cnidaria: Myxozoa: Myxosporea) in commercial fishes from Java Sea, Indonesia, with records of Unicapsula pyramidata and two new Kudoa spp. (2021)
  • Author(s): Yunus Muchammad、Yustinasari Lita Rakhma、Natalia Diana、Ghosh Subarna、Sakuma Kaishi、Inoue Ken、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 120 Issue: 3 Pages: 861-876
  • DOI: 10.1007/s00436-020-07030-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of a new species, Unicapsula aequilobata n. sp., and Unicapsula seriolae (Myxozoa: Myxosporea: Multivalvulida) in carangid fish from the South China Sea (2021)
  • Author(s): Inoue Ken、Li Ying-Chun、Ghosh Subarna、Yunus Muchammad、Zhang Jin-Yong、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 120 Issue: 7 Pages: 2379-2389
  • DOI: 10.1007/s00436-021-07108-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phylogenetic characterisation of seven Unicapsula spp. (Myxozoa: Myxosporea: Multivalvulida) from commercial fish in southern China and Japan (2020)
  • Author(s): Li Ying-Chun、Tamemasa Souhei、Zhang Jin-Yong、Sato Hiroshi
  • Journal Title: Parasitology Volume: 147 Issue: 4 Pages: 448-464
  • DOI: 10.1017/s0031182019001793
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phylogenetic relationships of three Kudoa spp. with morphologically similar myxospores (K. iwatai, K. lutjanus, and K. bora), with the redescription of K. uncinata and K. petala and description of a new species (K. fujitai n. sp.) in fishes in the South China Sea (2020)
  • Author(s): Li Ying-Chun、Inoue Ken、Zhang Jin-Yong、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 119 Issue: 4 Pages: 1221-1236
  • DOI: 10.1007/s00436-020-06636-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of four new Kudoa spp. (Myxozoa: Myxosporea: Multivalvulida) in commercial fishes collected from South China Sea, Atlantic Ocean, and Bering Sea by integrated taxonomic approach (2020)
  • Author(s): Li Ying-Chun、Inoue Ken、Tanaka Shuhei、Zhang Jin-Yong、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 119 Issue: 7 Pages: 2113-2128
  • DOI: 10.1007/s00436-020-06707-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphological and molecular characterization of a new species, Agglomerata daphniae n. sp. from the hypoderm of Daphnia magna (Crustacea: Daphniidae) (2020)
  • Author(s): Weng Meiqi、Liu Xinhua、Zhao Yuanli、Xie Derong、Zhang Qianqian、Sato Hiroshi、Zhang Jinyong
  • Journal Title: Journal of Invertebrate Pathology Volume: 177 Pages: 107501-107501
  • DOI: 10.1016/j.jip.2020.107501
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Synopsis of the species of the genus Zschokkella Auerbach, 1910 (Myxozoa: Bivalvulida: Myxidiidae) (2020)
  • Author(s): Matsche Mark A.、Yurakhno Violetta、Zhang Jinyong、Sato Hiroshi
  • Journal Title: Systematic Parasitology Volume: 98 Issue: 1 Pages: 25-55
  • DOI: 10.1007/s11230-020-09960-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integrated taxonomic approaches to seven species of capillariid nematodes (Nematoda: Trichocephalida: Trichinelloidea) in poultry from Japan and Indonesia, with special reference to their 18S rDNA phylogenetic relationships. (2020)
  • Author(s): Sakaguchi S, Yunus M, Sugi S, Sato H
  • Journal Title: Parasitol Res Volume: 119 Pages: 957-972
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phylogenetic characterisation of seven Unicapsula spp. (Myxozoa: Myxosporea: Multivalvulida) from commercial fish in southern China and Japan. (2020)
  • Author(s): Li Y-C, Tamemasa S, Zhang J-Y, Sato H
  • Journal Title: Parasitology Volume: 147 Pages: 448-464
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phylogenetic relationships of three Kudoa spp. with morphologically similar myxospores (K. iwatai, K. lutjanus, and K. bora), with the redescription of K. uncinata and K. petala and description of a new species (K. fujitai n. sp.) in fishes in the South China Sea. (2020)
  • Author(s): Li Y-C, Tamemasa S, Zhang J-Y, Sato H
  • Journal Title: Parasitol Res Volume: 119 Pages: 1221-1236
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integrative taxonomic approach of trypanosomes in the blood of rodents and soricids in Asian countries, with the description of three new species. (2019)
  • Author(s): Mafie E, Saito-Ito A, Kasai M, Hatta M, Rivera PT, Ma XH, Chen ER, Sato H, Takada N
  • Journal Title: Parasitol Res Volume: 118 Pages: 97-109
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New host records of three Kudoa spp. (K. yasunagai, K. thalassomi, and K. igami) with notable variation in the number of shell valves and polar capsules in spores. (2019)
  • Author(s): Sakai H, Kawai T, Zhang J, Sato H
  • Journal Title: Parasitol Res Volume: 118 Pages: 143-157
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] RNA-seq analysis of local tissue of Carassius auratus gibelio with pharyngeal myxobolosis: Insights into the pharyngeal mucosal immune response in a fish-parasite dialogue. (2019)
  • Author(s): Zhao Y, Liu X, Sato H, Zhang Q, Li A, Zhang J
  • Journal Title: Fish and Shellfish Immunology Volume: 94 Pages: 99-112
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Outbreak of trypanosomiasis in net-cape cultured barramundi, Lates calcarifer (Perciformes, Latidae), associated with Trypanosoma epinepheli (Kinetoplastida) in South China Sea. (2019)
  • Author(s): Luo D, Xu LW, Liu XH, Sato H, Zhang J
  • Journal Title: Aquaculture Volume: 501 Pages: 219-223
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphological and molecular characterization of the muscle-infecting myxosporean Myxobolus xinyangensis sp. nov. from Abbottina rivularis in China. (2019)
  • Author(s): Wu W, Wang QS, Sato H, Zhang JY
  • Journal Title: Dis Aquat Organ Volume: 132 Pages: 171-179
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphological and molecular-genetic characterization of Chloromyxum trilineatum n. sp. (Myxosporea: Bivalvulida) in the gall bladder of pale chub (Zacco platypus) in Japan. (2019)
  • Author(s): Sekiya M, Rosyadi I, Zhang JY, Sato H
  • Journal Title: Parasitol Res Volume: 118 Pages: 3349-3357
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First record of Trypanosoma dionisii of the T. cruzi clade from the Eastern bent-winged bat (Miniopterus fuliginosus) in the Far East (2018)
  • Author(s): Mafie Eliakunda、Rupa Fatema Hashem、Takano Ai、Suzuki Kazuo、Maeda Ken、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 117 Issue: 3 Pages: 673-680
  • DOI: 10.1007/s00436-017-5717-2
  • Peer Reviewed
• [Journal Article] Morphological and molecular genetic characterization of Kudoa konishiae n. sp. (Myxosporea: Multivalvulida) in the muscle of Japanese Spanish mackerel (Scomberomorus niphonius) (2018)
  • Author(s): Sakai Haruya、Kato Eigo、Sakaguchi Seiho、Setsuda Aogu、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 117 Issue: 3 Pages: 893-904
  • DOI: 10.1007/s00436-018-5770-5
  • Peer Reviewed
• [Journal Article] Molecular genetic diversity of Gongylonema neoplasticum (Fibiger & Ditlevsen, 1914) (Spirurida: Gongylonematidae) from rodents in Southeast Asia (2018)
  • Author(s): Setsuda Aogu、Ribas Alexis、Chaisiri Kittipong、Morand Serge、Chou Monidarin、Malbas Fidelino、Yunus Muchammad、Sato Hiroshi
  • Journal Title: Systematic Parasitology Volume: 95 Issue: 2-3 Pages: 235-247
  • DOI: 10.1007/s11230-018-9778-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An unexpected case of a Japanese wild boar (Sus scrofa leucomystax) infected with the giant thorny-headed worm (Macracanthorhynchus hirudinaceus) on the mainland of Japan (Honshu) (2018)
  • Author(s): Kamimura Koichiro、Yonemitsu Kenzo、Maeda Ken、Sakaguchi Seiho、Setsuda Aogu、Varcasia Antonio、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 117 Issue: 7 Pages: 2315-2322
  • DOI: 10.1007/s00436-018-5922-7
  • Peer Reviewed
• [Journal Article] Integrative taxonomic approach of trypanosomes in the blood of rodents and soricids in Asian countries, with the description of three new species (2018)
  • Author(s): Mafie Eliakunda、Saito-Ito Atsuko、Kasai Masatoshi、Hatta Mochammad、Rivera Pilarita T.、Ma Xiao-Hang、Chen Eng-Rin、Sato Hiroshi、Takada Nobuhiro
  • Journal Title: Parasitology Research Volume: 118 Issue: 1 Pages: 97-109
  • DOI: 10.1007/s00436-018-6120-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Gongylonema infection of wild mammals in Japan and Sardinia (Italy) (2018)
  • Author(s): Setsuda A.、Varcasia A.、Scala A.、Ozawa S.、Yokoyama M.、Torii H.、Suzuki K.、Kaneshiro Y.、Corda A.、Dess? G.、Tamponi C.、Cabras P.A.、Sato H.
  • Journal Title: Journal of Helminthology Volume: - Pages: 1-8
  • DOI: 10.1017/s0022149x18001001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New host records of three Kudoa spp. (K. yasunagai, K. thalassomi, and K. igami) with notable variation in the number of shell valves and polar capsules in spores (2018)
  • Author(s): Sakai Haruya、Kawai Takao、Zhang Jinyong、Sato Hiroshi
  • Journal Title: Parasitology Research Volume: 118 Issue: 1 Pages: 143-157
  • DOI: 10.1007/s00436-018-6144-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparative genomics of the major parasitic worms (2018)
  • Author(s): Coghlan A, Tyagi R, Cotton JA, Holroyd N, Rosa BA, Tsai IJ, Laetsch DR, Beech RN, Day TA, Hallsworth-Pepin K, Ke H-M, Kuo T-H, Lee TJ, Martin J, (60名共著者名省略), Sato H, Schnyder M, Scholz T, Shafie M, Tanya VN, Toledo R, Tracey A, Urban JF, Wang L-C, Zarlenga D, Blaxter ML, Mitreva M, Berriman M
  • Journal Title: Nature Genetics Volume: 51 Issue: 1 Pages: 163-174
  • DOI: 10.1038/s41588-018-0262-1
  • Peer Reviewed / Int'l Joint Research
• [Presentation] First report of three multivalvulid species （Cnidaria： Myxozoa： Myxosporea） in commercial fishes from Java Sea, Indonesia (2020)
  • Author(s): Muchammad Yunus、Lita Rakhma Yustinasari、Diana Natalia、Subarna Ghosh、Kaishi Sakuma、Ken Inoue、Aogu Setsuda、Hiroshi Sato
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] 南シナ海産のアジ科市販魚（マルアジおよびホソヒラアジ）から得られた未記載Unicapsula sp.とUnicapsula seriolaeについて (2020)
  • Author(s): 井上 健、李 迎春、Subarna Ghosh、章 晋勇、佐藤 宏
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] 多殻目Unicapsula属粘液胞子虫の分子系統学的種鑑別のための基盤研究. (2019)
  • Author(s): 李 迎春, 為政草平, 上村耕一郎, 章 晋勇, 柳田哲矢, 佐藤 宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] シスト形成クドア３種 (Kudoa iwatai, K. bora, K. lutjanus) 独立性の検証 ー広東省湛江市で市販される海産魚調査からー. (2019)
  • Author(s): 李 迎春, 佐藤 宏
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] 多殻目Unicapsula属粘液胞子虫の分子系統学的種鑑別のための基盤研究 (2019)
  • Author(s): 李 迎春、為政草平、上村耕一郎、章 晋勇、柳田哲矢、佐藤 宏
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] 多殻目Unicapsula属粘液胞子虫の種多様性: 広東省湛江市で市販される海産魚調査から (2018)
  • Author(s): 李 迎春、佐藤 宏
  • Organizer: 第161回日本獣医学会学術大会