| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Insulin receptor (IR) pre-mRNA undergoes alternative splicing to produce two isoforms, IR-A and B. We prepared a splicing reporter that contains a portion of rat IR gene. We confirmed our reporter recapitulate endogenous IR splicing pattern in rat H4IIE cells, and deletion analyses identified a binding site for splicing regulator Rbfox. We further demonstrate that SRSF3 and Rbfox2 are required for IR-B type splicing in H4IIE cells. Finally, we found that amino acid depletion causes IR splicing change, indicating that amino acid has a novel role as a metabolic regulatory signal.
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