| Project/Area Number |
18K06026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | レシピエント / 胚移植 / 近交系 / レシピエントマウス / 偽妊娠マウス |
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| Outline of Final Research Achievements |
As a recipient mouse for embryo transfer, it is common to use the ICR strain. The ICR strain cannot be produced in-house on a small scale, and its period of use is limited due to obesity. We have developed inbred-derived recipient mice to replace the closed colony ICR strain. Albino inbred KS mice rarely required cesarean section after embryo transfer, which made it possible to save labor and reduce the number of animals used. The weaning rate when frozen and thawed fertilized eggs of the C57BL/6 strain, which are often used in the production of genetically modified mice, was transplanted intraoductally did not decrease compared to the results when directly transplanted without freezing after egg collection. These show the excellent properties of KS mice as embryo transfer recipient mice.
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| Academic Significance and Societal Importance of the Research Achievements |
遺伝子改変マウスを作成するには，受精卵を偽妊娠させた受容雌マウス（レシピエント）に移植して産仔を得る必要がある。レシピエントに使用されるICR系統は遺伝的に均一ではないことから，肥満により使用期間が短いことや，帝王切開して里親に哺育させることが多いなど，大規模なコロニーを維持する必要がある。そこで作業効率の向上を目指し，小規模に自家繁殖して効率よく使用できるレシピエントとして，アルビノ近交系のKSマウスを開発した。KS系統を用いれば小規模に胚移植を行えるので，遺伝子改変マウスを用いる研究の普及に加え，動物使用数削減にも貢献できる。
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