Elucidation of muscular atrophy mechanism by optogenetics oxidative stress induction method and search for therapeutic agents
| Project/Area Number |
18K06029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kyoto University |
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Principal Investigator |
Sato Fuminori 京都大学, ウイルス・再生医科学研究所, 特定助教 (10588263)
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| Co-Investigator(Kenkyū-buntansha) |
瀬原 淳子 京都大学, ウイルス・再生医科学研究所, 連携教授 (60209038)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゼブラフィッシュ / 骨格筋 / 筋萎縮 / 酸化ストレス / ミトコンドリア / オプトジェネティクス / zebrafish / ミトコンドリ / オプトジェネティスク |
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| Outline of Final Research Achievements |
The purpose of this study is to analyze the process and mechanism by which oxidative stress in skeletal muscle leads to muscular atrophy. Therefore, it is essential to investigate how mitochondrial disorders (overproduction of ROS) in skeletal muscle affect skeletal muscle while maintaining a complex system using Individual organism, and analyze the muscular atrophy process caused.
An attempt was made to establish the above experimental system using zebrafish, but it was not possible to establish a transgenic zebrafish line that expresses a protein that produces ROS by light irradiation in skeletal muscle mitochondria. In order to carry out the research, it is necessary to introduce an expression control system that can control expression control more strictly and to establish a transgenic zebrafish strain again.
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| Academic Significance and Societal Importance of the Research Achievements |
酸化ストレスによる骨格筋萎縮を生体内のミトコンドリア特異的なROS産生誘導により解析した研究はこれまでほとんどなく、さらにROS産生の誘導方法として光照射を用い、実験対象動物としてゼブラフィッシュを用いたものはない。
新たな骨格筋萎縮機構の解明に貢献するだけでなく、高効率な治療薬スクリーニングなどへの流用も可能である。
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Report
(4 results)
Research Products
(6 results)
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[Presentation] 人工重力発生装置を用いたゼブラフィッシュ宇宙滞在実験2018
Author(s)
佐藤文規, Choi Minyong, 王梓, 今村聖実, 堀内映美, 呉泉, 藤田生水, 内田智子, 加藤充康, 谷垣文章, 東端晃, 村谷匡史, 小林純也, 高橋昭久, 菅野純夫, 松崎文雄, 鈴木穣, 川上浩一, 瀬原淳子
Organizer
宇宙生物科学会第32回大会
Related Report
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[Presentation] ゼブラフィッシュ宇宙滞在実験から微小重力の骨格筋への影響とそのメカニズムを探る2018
Author(s)
佐藤文規, Choi Minyong, 王梓, 今村聖実, 堀内映美, 呉泉, 藤田生水, 内田智子, 加藤充康, 谷垣文章, 東端晃, 村谷匡史, 小林純也, 高橋昭久, 菅野純夫, 松崎文雄, 鈴木穣, 川上浩一, 瀬原淳子
Organizer
第41回日本分子生物学会年会
Related Report
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[Presentation] Study on gravity-dependent skeletal muscle maintenance mechanisms using Zebrafish, aquatic organisms2018
Author(s)
Fuminori Sato, Minyong Choi, Zi Wang, Kiyomi Imamura, Terumi Horiuchi, Wu Quan, Ikumi Fujita, Satoko Uchida, Mitsuyasu Kato, Fumiaki Tanigaki, Akira Higashibata, Masafumi Muratani, Junya Kobayashi, Akihisa Takahashi, Sumio Sugano, Fumio Matsuzaki, Yutaka Suzuki, Koichi Kawakami, Atsuko Sehara-Fujisawa
Organizer
2018 ASCB | EMBO Annual Meeting
Related Report
Int'l Joint Research
