Analysis of mechanism to induce potent neutralizing antibodies using SIV-infected macaques
| Project/Area Number |
18K06032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
Kuwata Takeo 熊本大学, ヒトレトロウイルス学共同研究センター, 特定事業教員 (70346063)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 中和抗体 / SIV / 遺伝子多型 / アカゲザル / 抗体 / エイズ |
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| Outline of Final Research Achievements |
Analysis of induction of B404-class antibodies, which neutralize various SIV strains, was performed using 6 SIV-infected macaques. Induction of B404-class antibodies was associated with polymorphism of immunoglobulin Heavy chain gene, and macaques with the VH3.33 allele with E at 38th position and T at 65th position induced B404-class antibodies. The 38E was important for binding to Env and neutralizing activity. These results suggest that induction of neutralizing antibodies is determined by polymorphism of immunoglobulin genes.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、多様なHIV-1株を広範に中和するbnAbの誘導が効果的なワクチンの開発に重要であると考えられるようになった。また、特定のbnAbを誘導するための抗原の開発も進んでいる。しかしながら、HIV-1感染によるbnAbの誘導は稀であり、そのメカニズムも不明である。本研究では、SIV感染サル・モデルを用いて中和抗体誘導の解析を行い、中和抗体の誘導と抗体遺伝子の多型が密接に関連していることを示した。この結果は、今後のワクチン開発に遺伝子多型を考慮する必要があることをあきらかにした。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Existence of replication-competent minor variants with different coreceptor usage in plasma from HIV-1-infected individuals2020
Author(s)
Maeda Y, Takemura T, Chikata T, Kuwata T, Terasawa H, Fujimoto R, Kuse N, Akahoshi T, Murakoshi H, Van Tran G, Zhang Y, Pham CH, Pham AHQ, Monde K, Sawa T, Matsushita S, Nguyen TV, Van Nguyen K, Hasebe F, Yamashiro T, Takiguchi M.
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Journal Title
Journal of Virology
Volume: 94
Issue: 12
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Synergistic inhibition of cell-to-cell HIV-1 infection by combinations of single chain variable fragments and fusion inhibitors2019
Author(s)
Mamun MA, Kuwata T, Tanaka K, Alam M, Takahama S, Shimura K, Matsuoka M, Fukuda N, Morioka H, Tamamura H, Matsushita S
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Journal Title
Biochemistry and biophysics reports
Volume: 20
Pages: 100687-100687
Related Report
Peer Reviewed
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