Bovine leukemia virus infection as a model of HTLV-1 -Dok genes function-
| Project/Area Number |
18K06036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
Ohsugi Takeo 酪農学園大学, 獣医学群, 教授 (00211102)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ウシ / 牛白血病 / DOK1遺伝子 / DOK2遺伝子 / DOK3遺伝子 / HTLV-1 / BLV / ＤＯＫ１遺伝子 / ＤＯＫ２遺伝子 / ＤＯＫ３遺伝子 / ＨＴＬＶ－１ / DOk3遺伝子 / ＢＬＶ / 牛 / Dok / animal model |
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| Outline of Final Research Achievements |
Downstream of tyrosine kinase (DOK) family molecules act suppressively on cancer development. Therefore, we also searched for the expression of the DOK gene in the blood cells of healthy cattle, which has not been well known, and the expression in bovine leukemia tumors and their tumor lines. In healthy bovine blood cells, DOK1 / 2 was expressed in T cells and DOK1 / 3 was expressed in B cells, which was similar to the report in mice. On the other hand, the expression level of the DOK3 gene in the bovine leukemia cell line KU-1, which is derived from B cells, was more than 4-fold higher than that in normal B cells. The DOK3 gene in field bovine lymphoma was also expressed about twice as high as normal B cells on average. On the other hand, the expression of the DOK1 / 2 gene was the same as that of healthy cattle.
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| Academic Significance and Societal Importance of the Research Achievements |
DOK1/2/3遺伝子は血液細胞に発現し白血病の発生を抑制すると考えられている。健常牛ではB細胞では、DOK/3の発現が認められるが、牛白血病を発生するとさらにその発現が増加する。本来DOK3はがん発生を抑制するはずであるが、その抑制機構が暴走している可能性がある。この機構を探索することにより牛白血病の発症のメカニズムを解明できる可能性がある。
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] A novel mouse model of adult T‐cell leukemia cell invasion into the spinal cord.2019
Author(s)
Ohsugi, T., Tanaka, S., Iwasaki, K., Nagano, Y., Kozako, T., Matsuda, K., Hirose, T. and Takehana, K.
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Journal Title
Animal Model Exp. Med.
Volume: 2
Issue: 1
Pages: 64-67
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] New SIRT2 inhibitors: Histidine-based bleomycin spin-off2019
Author(s)
Ali Taha F.S.、Ciftci Halil I.、Radwan Mohamed O.、Koga Ryoko、Ohsugi Takeo、Okiyama Yoshio、Honma Teruki、Nakata Akiko、Ito Akihiro、Yoshida Minoru、Fujita Mikako、Otsuka Masami
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Journal Title
Bioorganic & Medicinal Chemistry
Volume: 27
Issue: 9
Pages: 1767-1775
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas2019
Author(s)
Yamagishi M., Hori M., Fujikawa D., Ohsugi T., Honma D., Adachi N., Katano H., Hishima T., Kobayashi S., Nakano K., Nakashima M., Iwanaga M., Utsunomiya A., Tanaka Y., Okada S., Tsukasaki K., Tobinai K., Araki K., Watanabe T., Uchimaru K.
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Journal Title
Cell Reports
Volume: 29
Issue: 8
Pages: 2321-2337
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines.2018
Author(s)
Kozako, T., Mellini, P., Ohsugi, T., Aikawa, A., Uchida, Y. I., Honda, S. I., Suzuki, T.
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Journal Title
BMC Cancer
Volume: 18
Issue: 1
Pages: 791-791
DOI
Related Report
Peer Reviewed / Open Access
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