| Project/Area Number |
18K06038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Discontinued (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | コンティショナルレスキュー / コンディショナルノックアウトマウス / Inv/ inversin / 左右軸形成 / 多発性嚢胞腎 / kit ligand / 幹細胞 / 疾患モデルマウス / コンティショナルレスキュー法 / inversin 遺伝子 / c-kit Sl 遺伝子 / disease model animal / transgenic rescue mice / conditional knockout / inversin / polycystic kidney / cilia / left/light asymmetry / c-kit / transgenic rescue / Inversin / transgenic mice / model animal |
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| Outline of Final Research Achievements |
Although the conditional knockout mouse method is effective for analyzing tissue-specific gene functions, it requires special techniques and a lot of time because ES cells are necessary for its generation. Therefore, we developed a conditional rescue technique (Patent No. 6327712) that does not require ES cells and is more convenient for analysis of gene function. In this study, in addition to the Inv gene, we generated novel conditional rescue mice target for the kit ligand genes, which play important roles in stem cell proliferation and migration, using a second-generation vector system that enables real-time observation of the switching of rescue gene expression. We have shown that these novel technologies are very effective for the analysis of the tissue- and development-specific gene function.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究においては新規のベクターを用いて、Inv遺伝子のほか新たにkit ligand遺伝子に対するコンディショナルレスキューマウスの作成に成功し、我々が開発した手法が多くの遺伝子の機能解析において有効的に機能することを明らかにした. 本手法は、従来の手法と比べ、短時間かつ低コストで目的とする遺伝子の組織または細胞特異的な欠失の誘導が可能となる新規の手法である.さらにこの手法は ES 細胞を必要としないため、ES 細胞が存在しないマウス系統やマウス以外の動物種にも適応可能である. 本手法は今後遺伝子の機能解析に広く適用されることが期待される．
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