| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To analyze molecular mechanisms, transgene induction is one of the most popular approach in biology field. Well-characterized promoters can induce the downstream gene of interest. Such promoters were established by previous studies, but systematic approaches have not been applied to identify the best candidates for it. In this study, transcriptome data was utilized for the identification of the candidate that could induce downstream genes.
The identified gene promoters in this study retained a significant transcriptional activity, but not superior active than CAG promoter that is classically used in this field. These observations suggest that structure of promoter is more important than sequence themselves.
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