| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
Genetic analysis revealed that (1) overexpression of Mpt5 had a growth inhibitory effect specifically in the taf1-deltaTAND strain, (2) overexpression of Mpt5[5A mutant] and Ssd1 had a growth inhibitory effect in the presence or absence of TAND, and (3) the growth inhibitory effect of Mpt5 overexpression was significantly increased in the ssd1-d strain. These results suggest that the synthetic lethality of the delta-RAM ssd1-d taf1-deltaTAND triple mutant strain is due to the up-regulation of Mpt5 function. RNA-seq analysis also provided clues to elucidate the cause of synthetic lethality in this triple mutant strain from the aspect of gene expression profiles.
|
