Analysis of an allergen of cypress BP14 involved in pollen food associated syndrome

• Project/Area Number: 18K06074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43020:Structural biochemistry-related
• Research Institution: Hokkaido University
• Principal Investigator: AIZAWA Tomoyasu 北海道大学, 先端生命科学研究院, 教授 (40333596)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アレルゲン / 花粉 / 果実 / GRP / BP14 / Cup s 7 / ペプチド / 立体構造 / Cry j 7 / NMR

Outline of Final Research Achievements

We proceeded with the study of an allergenic protein, BP14 derived from European cypress. BP14 is thought to be the cause of pollen-food allergy syndrome (PFAS).
We succeeded in identifying the BP14 gene by analysis with a next-generation sequencer. This sequence was registered as an allergen Cup s 7 in the WHO / IUIS database. By using recombinant Cup s 7 protein, the reactivity with patient serum was examined, and the three-dimensional structure was analyzed by the NMR method.

Academic Significance and Societal Importance of the Research Achievements

本研究で得られたBP14(Cup s 7)に関する知見は、新規診断技術の開発や花粉症・食物アレルギーの治療への応用にもつながる基礎研究となることが期待される。花粉症が原因となって引き起こされる食物アレルギーであるPFASのうち、ヒノキ科植物が関連するものについては、特に未知の点が多い。また、PFASの研究には、原因蛋白質の詳細な立体構造解析は重要な基礎情報となるが、その立体構造解析例は極めて少ない。このため、本研究の成果はヒノキ科植物のPFAS研究の進展だけでなく、PFASそのものに関する基礎、臨床両研究分野においても重要な意義を持つ。

Research Products

• [Int'l Joint Research] パスツール研究所(フランス)
• [Journal Article] Gibberellin-regulated protein sensitization in Japanese cedar (Cryptomeria japonica) pollen allergic Japanese cohorts (2021)
  • Author(s): Iizuka T, Takei M, Saito Y, Rumi F, Zheng J, Lu X, Chafey P, Broussard C, Guilloux-Assalet L, Charpin D, Ebisawa M, Senechal H, Aizawa T, Poncet P.
  • Journal Title: Allergy Volume: - Issue: 7 Pages: 14816-14816
  • DOI: 10.1111/all.14816
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ホソイトスギ由来花粉関連食物アレルギー症候群原因抗原Cup s 7の解析 (2020)
  • Author(s): 相沢智康
  • Journal Title: アレルギーの臨床 Volume: 40 Pages: 150-153
• [Journal Article] The subtype of Cupressaceae pollinosis associated with Pru p 7 sensitization is characterized by a sensitization to a cross-reactive gibberellin-regulated protein in cypress pollen: BP14. (2019)
  • Author(s): Poncet P, Aizawa T, Senechal H.
  • Journal Title: Clin Exp Allergy Volume: 49 Issue: 8 Pages: 1163-1166
  • DOI: 10.1111/cea.13418
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pollen/Fruit Syndrome: Clinical Relevance of the Cypress Pollen Allergenic Gibberellin-Regulated Protein. (2019)
  • Author(s): Senechal H, Keykhosravi S, Couderc R, Selva MA, Shahali Y, Aizawa T, Busnel JM, Arif R, Mercier I, Pham-Thi N, Charpin DA, Poncet P.
  • Journal Title: Allergy Asthma Immunol Res Volume: 11 Issue: 1 Pages: 143-151
  • DOI: 10.4168/aair.2019.11.1.143
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Gene Identification Of An Allergen Peptide From Cypress Pollen Using Next Generation Sequencer And Its Over Expression For Analysis (2020)
  • Author(s): Tomona Iizuka, Helene Senechal, Pascal Poncet, Tomoyasu Aizawa
  • Organizer: 第20 回日本蛋白質科学会年会