| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
A bacterial flagellar motor is a supramolecular complex that functions as a rotary motor consisting of a rotor and a stator. The energy for torque generation is mediated by an inward ion flux through the channels of the stator complex. Here, to clarify the structure of sodium-channel MotPS that acts as a Bacillus subtilis flagellar stator and its dynamic structural changes with high resolution, cryo-electron microscopy and high-speed AFM observation of the purified stator complex were carried out. We tested the chemically modified grids to achieve more effective ice embedding. More monodisperse particles were observed in one modified grid. On the other hand, high-speed AFM observation revealed that the transmembrane regions of MotPS stator complex strongly interact with the mica surface. Further improvements are necessary to control the orientation.
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