• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the operating mechanism of BepA involved in the biosynthesis of bacterial outer membrane LPS translocon

Research Project

Project/Area Number 18K06136
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43030:Functional biochemistry-related
Research InstitutionYamagata Prefectural Yonezawa University of Nutrition Sciences (2020)
Morioka College (2018-2019)

Principal Investigator

Narita Shin-ichiro  山形県立米沢栄養大学, 健康栄養学部, 教授 (30338751)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞内タンパク質分解 / 表層ストレス応答 / 細菌細胞表層 / プロテアーゼ / シャペロン / 外膜タンパク質
Outline of Final Research Achievements

Outer membrane of gram-negative bacteria contains β-barreled outer membrane proteins. An Escherichia coli periplasmic protein BepA contributes to the maintenance of the structure and quality of the outer membrane by promoting the assembly of outer membrane proteins through its chaperone-like activity, and by degrading and removing abnormal outer membrane proteins through its protease activity. To elucidate the mechanism by which BepA discriminate between outer membrane proteins to be assembled and those to be degraded, we constructed BepA mutants and analyzed their function. We found that the α9/H246 loop located near the protease active site of BepA normally suppresses the protease activity of BepA, and that the reversible conformational change of this loop induces the protease activity of BepA to degrade outer membrane proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究では、大腸菌プロテアーゼによる外膜の機能維持の分子機構をアミノ酸残基レベルで解明した。ヒスチジン残基を介してプロテアーゼ活性が可逆的に制御されるという新たな活性調節機構を解明したことにより、細胞内タンパク質の品質維持においてプロテアーゼとシャペロンの働きがどのように制御されているかという、大腸菌からヒトまで共通する普遍的なテーマに対して新たな知見を提供することができた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Remarks (4 results)

  • [Journal Article] Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.2020

    • Author(s)
      Daimon 他7名
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 117 Issue: 45 Pages: 27989-27996

    • DOI

      10.1073/pnas.2010301117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural basis of the function of the β-barrel assembly-enhancing protease BepA2019

    • Author(s)
      Mohammad Shahrizal, Yasushi Daimon, Yoshiki Tanaka, Yugo Hayashi, Shintaro Nakayama, Shigehiro Iwaki, Shin-ichiro Narita, Hironari Kamikubo, Yoshinori Akiyama, Tomoya Tsukazaki
    • Journal Title

      Journal of Molecular Biology

      Volume: 431 Issue: 3 Pages: 625-635

    • DOI

      10.1016/j.jmb.2018.11.024

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Remarks] BepAによるβ-バレル型タンパク質の選択的分解を支える可逆的な自己活性抑制機構

    • URL

      https://sites.google.com/view/yunsnarita/%E6%8A%84%E8%A8%B3

    • Related Report
      2020 Annual Research Report
  • [Remarks] Reversible auto-inhibitory regulation of BepA

    • URL

      https://www.biorxiv.org/content/10.1101/2020.07.07.192476v1

    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究テーマ

    • URL

      https://sites.google.com/view/yunsnarita/home/yan-jiutema

    • Related Report
      2019 Research-status Report
  • [Remarks] 研究テーマ

    • URL

      https://sites.google.com/site/mununarita/home/yan-jiutema

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-12-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi