A crosstalk between membrane trafficking and cytoskeletons in invadopodia in cancer cells
| Project/Area Number |
18K06138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
Inoue Hiroki 東京薬科大学, 生命科学部, 講師 (10294448)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 浸潤突起 / 微小管 / アクチン / 小胞輸送 / 細胞外基質 / がん / 浸潤 / 転移 / アクチン線維 |
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| Outline of Final Research Achievements |
Invadopodia in invasive cancer cells are actin-enriched structures with an ability to degrade extracellular matrix (ECM) and play crucial roles in invasion and metastasis. A formation of functional invadopodia may require a crosstalk between microtubules and actin filaments, but the molecular mechanisms are not fully understood. In addition, regulatory mechanisms of the polarized transport of metalloproteinases degrading ECM at invadopodia remain elusive. In this study, we have focused on the microtubule-associated protein (MAP) that is associated with the crosstalk between microtubules and actin filaments and the SNARE proteins that function in the transport of metalloproteinases to invadopodia and revealed a part of the molecular mechanisms of invadopodia formation followed by cancer metastasis.
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| Academic Significance and Societal Importance of the Research Achievements |
がんによる死因の90％は、がん細胞の浸潤・転移が関係している。乳がんにおいても、転移の無い場合の5年生存率は99%だが、転移すると27%に低下する。本研究は、悪性度の高い乳がん細胞の浸潤転移に関わる機構の一端を分子レベルで解明したものであり、学術的な意義にとどまらず、新規の診断・治療標的として利用できる可能性も秘めている。
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane.2019
Author(s)
Miyagawa, T.,* Hasegawa, K.,* Aoki, Y.,* Watanabe, T.,* Otagiri, Y., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Yamaguchi, H., Tagaya, M. and Inoue, H. (*, These authors contributed equally to this work.)
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Journal Title
J. Cell Biol.
Volume: 218
Issue: 10
Pages: 3355-3371
DOI
Related Report
Peer Reviewed / Open Access
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