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Imaging of cytoplasmic dynein structural status

Research Project

Project/Area Number 18K06147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43040:Biophysics-related
Research InstitutionThe University of Tokyo

Principal Investigator

Shima Tomohiro  東京大学, 大学院理学系研究科(理学部), 助教 (60631786)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords自己阻害 / 蛍光共鳴エネルギー移動 / 細胞内輸送 / 運動活性制御 / AAA+ / モータータンパク質 / 微小管 / 細胞骨格 / 細胞骨格系モータータンパク質 / 自己制御 / ライブイメージング
Outline of Final Research Achievements

The appropriate intracellular transport is crucial for many biological functions in eukaryotes. For this purpose, the motor activities of kinesins and cytoplasmic dynein, which are responsible for transporting cargoes in opposite directions on microtubules, need to be properly regulated. Recently, Recently, a model has been proposed for the regulation of dynein activity by changing the orientation of the two motor domains in a single dynein molecule, but this has not yet been directly tested. In this study, we developed a method to measure the distance and orientation between the two motor domains by fluorescence resonance energy transfer (FRET), and obtained results suggesting that the distance between the motor domains varies with time and depends on the nucleotide state.

Academic Significance and Societal Importance of the Research Achievements

真核生物内でおこる多くの生命機能にとって、細胞内で物質が正しい方向へ輸送されることは非常に重要である。そのためには、微小管上においてそれぞれ逆方向への輸送を担っているキネシンと細胞質ダイニンの運動活性が適切に調節される必要がある。小胞の種類や輸送経路ごとに多種多様なキネシンが存在するのに対し、細胞質ダイニンは1種類でほぼすべての細胞質中での小胞輸送に対応しているため、細胞質ダイニンの活性調節が輸送方向決定の鍵を握ると考えられている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (23 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 7 results) Presentation (9 results) (of which Int'l Joint Research: 1 results) Book (3 results)

  • [Int'l Joint Research] Delft University of Technology(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Institut Jaques-Monod(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Delft University of Technology(オランダ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Institut Jacques-Monod(フランス)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking2020

    • Author(s)
      Ando J, Shima T, Kanazawa R, Shimo-Kon R, Nakamura A, Yamamoto M, Kon T, Iino R
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1080-1080

    • DOI

      10.1038/s41598-020-58070-y

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] How Cytoplasmic Dynein Couples ATP Hydrolysis Cycle to Diverse Stepping Motions: Kinetic Modeling2020

    • Author(s)
      Kubo Shintaroh、Shima Tomohiro、Takada Shoji
    • Journal Title

      Biophysical Journal

      Volume: 118 Issue: 8 Pages: 1930-1945

    • DOI

      10.1016/j.bpj.2020.03.012

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Vitro Reconstitution of Dynein Force Exertion in a Bulk Viscous Medium2020

    • Author(s)
      Palenzuela Heliciane、Lacroix Benjamin、Salle Jeremy、Minami Katsuhiko、Shima Tomohiro、Jegou Antoine、Romet-Lemonne Guillaume、Minc Nicolas
    • Journal Title

      Current Biology

      Volume: 30 Issue: 22 Pages: 4534-4540.e7

    • DOI

      10.1016/j.cub.2020.08.078

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Implementation of single molecule FRET for visualizing intramolecular movement in CRISPR-Cas92020

    • Author(s)
      Narita Haruka、Ebata Hiroshi、Sakai Karibu、Minami Katsuhiko、Uemura Sotaro、Shima Tomohiro
    • Journal Title

      bioRxiv

      Volume: 2020.04.13.039537 Pages: 1-28

    • DOI

      10.1101/2020.04.13.039537

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Open Access
  • [Journal Article] In Vitro Reconstitution of Dynein Force Exertion in Bulk Cytoplasm2020

    • Author(s)
      Palenzuela Heliciane、Lacroix Benjamin、Salle Jeremy、Minami Katsuhiko、Shima Tomohiro、Jegou Antoine、Romet-Lemonne Guillaume、Minc Nicolas
    • Journal Title

      bioRxiv

      Volume: 2020.05.06.081364

    • DOI

      10.1101/2020.05.06.081364

    • Related Report
      2019 Research-status Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Second harmonic generation polarization microscopy as a tool for protein structure analysis2019

    • Author(s)
      Kaneshiro Junichi、Okada Yasushi、Shima Tomohiro、Tsujii Mika、Imada Katsumi、Ichimura Taro、Watanabe Tomonobu M.
    • Journal Title

      Biophysics and Physicobiology

      Volume: 16 Issue: 0 Pages: 147-157

    • DOI

      10.2142/biophysico.16.0_147

    • NAID

      130007709947

    • ISSN
      2189-4779
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diverse stepping motions of cytoplasmic dynein revealed by kinetic modeling2019

    • Author(s)
      Shintaroh Kubo, Tomohiro Shima, Shoji Takada
    • Journal Title

      bioRxiv

      Volume: 516179 Pages: 1-34

    • DOI

      10.1101/516179

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Presentation] A fast and fully automated HMM fitting algorithm enables accurate analysis of biophysical data with numerous states2021

    • Author(s)
      Liu Hanjin、Shima Tomohiro、Uemura Sotaro
    • Organizer
      65th Biophysical Society Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a new hidden Markov model fitting algorithm without predefinition of parameters for objective biophysical data analysis2020

    • Author(s)
      Liu Hanjin、Shima Tomohiro、Uemura Sotaro
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] N-terminal region of Drosophila Argonaute2 can form amyloid fibrils2020

    • Author(s)
      Narita Haruka, Kuwabara Makoto F., Komori Tomotaka, Murakami Ryo, Shima Tomohiro, Siomi Mikiko C., Uemura Sotaro
    • Organizer
      第20回日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] N- terminal region of Drosophila Argonaute2 can form amyloid fibrils2019

    • Author(s)
      成田 晴香、 桑原 誠、小森 智貴、 村上 僚、島 知弘、塩見 美喜子、上村 想太郎
    • Organizer
      第19回日本蛋白質学会
    • Related Report
      2019 Research-status Report
  • [Presentation] N- terminal region of Drosophila Argonaute2 can form amyloid fibrils2019

    • Author(s)
      成田 晴香、 桑原 誠、小森 智貴、 村上 僚、島 知弘、塩見 美喜子、上村 想太郎
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Key residues on cytoplasmic dynein for asymmetric unbinding from microtubule2019

    • Author(s)
      Shintaroh Kubo、Tomohiro Shima、Takahide Kon、Shoji Takada
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Synthesis of fluorescent ATP to elucidate coordination of multiple ATPase sites in cytoplasmic dynein2018

    • Author(s)
      Karibu Sakai, Tomotaka Komori, Tomohiro Shima, Sotaro Uemura
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Single-particle tracking of motor domain of a processive dynein at microsecond time resolution and nanometer localization precision2018

    • Author(s)
      Jun Ando, Tomohiro Shima, Akihiko Nakamura, Akasit Visootsat, Mayuko Yamamoto, Takahide Kon, Ryota Iino
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Bi-pedal motions of cytoplasmic dynein via Markov state modeling2018

    • Author(s)
      Shintaroh Kubo, Tomohiro Shima, Shoji Takada
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Research-status Report
  • [Book] Make Life Visible2020

    • Author(s)
      Shima Tomohiro、Uemura Sotaro
    • Total Pages
      292
    • Publisher
      Springer, Singapore
    • Related Report
      2020 Annual Research Report
  • [Book] Make Life Visible2020

    • Author(s)
      Tomohiro Shima, Sotaro Uemura
    • Total Pages
      292
    • Publisher
      Springer Singapore
    • ISBN
      9789811379079
    • Related Report
      2019 Research-status Report
  • [Book] Handbook of Dynein (Second Edition)2019

    • Author(s)
      Tomohiro Shima, Takahide Kon
    • Total Pages
      420
    • Publisher
      CRC Press
    • ISBN
      9789814800013
    • Related Report
      2019 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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