Structural study on the function of chloride ion in photosystem II
| Project/Area Number |
18K06158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Jichi Medical University (2020)
Okayama University (2018-2019) |
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Principal Investigator |
Umena Yasufumi 自治医科大学, 医学部, ポスト・ドクター (10468267)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 光合成 / 結晶構造解析 / 分子アニオン / 塩素イオン / 水分解反応 / 結晶構造 / プロトン排出機構 / 蛋白質結晶構造解析 / 水分解反応の阻害因子 / 光合成蛋白質 / 蛋白質結晶 / X線結晶構造解析 / 反応阻害物質 |
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| Outline of Final Research Achievements |
In order to elucidate the role of chloride ion, which is an essential cofactor for water splitting in photosystem II (PSII) in photosynthesis reaction, we studied the inhibition mechanism from the crystal structure of PSII bound with molecular anions, which reduced PSII activity and to understand the original role of chloride ion from structural chemistry.
This study succeeded in analyzing PSII crystals substituted with molecular anions azide, nitrate, and nitrite ions at 2-2.1 angstrom resolution. The inhibition mechanism was clarified from the common structural changes associated with these substitutions compared with the original structure. We concluded that the role of the chlorine ion in PSII is for supporting the proton transfer mechanism and for sustaining the structure of the active center of the Mn-cluster.
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| Academic Significance and Societal Importance of the Research Achievements |
PSIIの水分解反応は金属触媒のMnクラスターを中心に議論がされているが、補欠因子としての塩素イオンの構造化学的な役割については、はっきりしていなかった。本研究により、PSIIにおける水分解反応をより包括的に議論できる構造情報を示すことができた。この知見は、Mnクラスターを模倣したMn触媒の周辺構造が重要性を提示することで、新たな人工光合成触媒への設計指針を提供できたものと思われる。
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Capturing structural changes of the S1 to S2 transition of photosystem II using time-resolved serial femtosecond crystallography2021
Author(s)
H Li, Y Nakajima, T Nomura, M Sugahara, S Yonekura, SK Chan, T Nakane, T Yamane, Y Umena, M Suzuki, T Masuda, T Motomura, H Naitow, Y Matsuura, T Kimura, K Tono, S Owada, Y Joti, R Tanaka, E Nango, F Akita, M Kubo, S Iwata, JR Shen and M Suga
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Journal Title
IUCrJ
Volume: 8
Issue: 3
Pages: 431-443
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] X-ray fluorescence holography for soft matter2019
Author(s)
Ang Artoni Kevin Roquero、Sato-Tomita Ayana、Shibayama Naoya、Umena Yasufumi、Happo Naohisa、Marumi Riho、Kimura Koji、Matsushita Tomohiro、AKAGI Kazuto、Sasaki Takahiko、Sasaki Yuji C.、Hayashi Kouichi
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Journal Title
Japanese Journal of Applied Physics
Volume: 59
Issue: 1
Pages: 010505-010505
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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