Studies on ion selectivity in cellular signaling

• Project/Area Number: 18K06159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: Morimoto Yusuke 九州工業大学, 大学院情報工学研究院, 准教授 (50631777)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: シグナル伝達 / 膜電位 / 蛍光イメージング / 走化性 / 光遺伝学 / イオン選択性 / 細胞運動 / 細胞分化

Outline of Final Research Achievements

Using the signal transduction mechanism of cellular slime mold as a model, we aimed to clarify the role of ion-selective membrane potential and cytoplasmic ion concentration changes in eukaryotic signal transduction. Simultaneous measurements of various biological phenomena and ion concentration fluctuations were performed to identify the calcium signaling pathway involved in mechanical stimulation of cellular slime mold. We also succeeded in detecting cytoplasmic pH changes during differentiation and dedifferentiation of cellular slime mold.

Academic Significance and Societal Importance of the Research Achievements

本研究で確立したイメージング技術は、細胞性粘菌の研究だけでなく、原核生物から真核生物に至るまでの幅広い研究に応用できるものである。実際に本課題において、バクテリアの計測に応用した研究によって成果が得られている。そのため、基礎生命科学から臨床研究までの幅広い分野での応用が期待される。

Research Products

• [Journal Article] Architecture and assembly of the bacterial flagellar motor complex. (2021)
  • Author(s): Morimoto, YV, Minamino T.
  • Journal Title: Subcell. Biochem. Volume: 96 Pages: 297-321
  • DOI: 10.1007/978-3-030-58971-4_8
  • ISBN: 9783030589707, 9783030589714
  • Peer Reviewed
• [Journal Article] The FlgN chaperone activates the Na+-driven engine of the Salmonella flagellar protein export apparatus (2021)
  • Author(s): Minamino T, Kinoshita M, Morimoto YV, Namba K.
  • Journal Title: Commun. Biol. Volume: 4 Issue: 1 Pages: 335-335
  • DOI: 10.1038/s42003-021-01865-0
  • Peer Reviewed / Open Access
• [Journal Article] GFP fusion to the N-terminus of MotB affects the proton channel activity of the bacterial flagellar motor in Salmonella. (2020)
  • Author(s): Morimoto YV, Namba K, Minamino T.
  • Journal Title: Biomolecules Volume: 10 Issue: 9 Pages: 1255-1255
  • DOI: 10.3390/biom10091255
  • Peer Reviewed / Open Access
• [Journal Article] Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements (2020)
  • Author(s): Kinosita Yoshiaki、Ishida Tsubasa、Yoshida Myu、Ito Rie、Morimoto Yusuke V.、Goto Kazuki、Berry Richard M.、Nishizaka Takayuki、Sowa Yoshiyuki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 15887-15887
  • DOI: 10.1038/s41598-020-72429-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds (2020)
  • Author(s): Shin-Ichi Yamazaki, Hidenori Hashimura, Yusuke V. Morimoto, Yukihiro Miyanaga, Satomi Matsuoka, Yoichiro Kamimura, Masahiro Ueda
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 525 Issue: 2 Pages: 372-377
  • DOI: 10.1016/j.bbrc.2020.02.060
  • Peer Reviewed / Open Access
• [Journal Article] Structure of the native supercoiled flagellar hook as a universal joint. (2020)
  • Author(s): Kato, T., Makino, F., Miyata, T., Horvath, P. & Namba, K.
  • Journal Title: Nature Commun. Volume: 113 Issue: 4 Pages: 755-765
  • DOI: 10.1111/mmi.14440
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Novel Insights into Conformational Rearrangements of the Bacterial Flagellar Switch Complex (2019)
  • Author(s): Sakai Tomofumi、Miyata Tomoko、Terahara Naoya、Mori Koichiro、Inoue Yumi、Morimoto Yusuke V.、Kato Takayuki、Namba Keiichi、Minamino Tohru
  • Journal Title: mBio Volume: 10 Issue: 2 Pages: 1-14
  • DOI: 10.1128/mbio.00079-19
  • NAID: 120006822482
  • Peer Reviewed / Open Access
• [Journal Article] Effect of the MotA (M206I) mutation on torque generation and stator assembly in the Salmonella H+-driven flagellar motor (2019)
  • Author(s): Yuya Suzuki, Yusuke V Morimoto, Kodai Oono, Fumio Hayashi, Kenji Oosawa, Seishi Kudo, Shuichi Nakamura
  • Journal Title: Journal of bacteriology Volume: 201 Issue: 6
  • DOI: 10.1128/jb.00727-18
  • Peer Reviewed
• [Journal Article] Collective cell migration of Dictyostelium without cAMP oscillations at multicellular stages (2019)
  • Author(s): Hidenori Hashimura, Yusuke V. Morimoto, Masato Yasui, Masahiro Ueda
  • Journal Title: Communications Biology Volume: 2 Issue: 1 Pages: 34-34
  • DOI: 10.1038/s42003-018-0273-6
  • Peer Reviewed / Open Access
• [Journal Article] A study of wound repair in Dictyostelium cells by using novel laserporation (2018)
  • Author(s): Mst Shaela Pervin, Go Itoh, Md Shahabe Uddin Talukder, Koushiro Fujimoto, Yusuke V Morimoto, Masamitsu Tanaka, Masahiro Ueda, Shigehiko Yumura
  • Journal Title: Scientific reports Volume: 8 Issue: 1 Pages: 7969-7969
  • DOI: 10.1038/s41598-018-26337-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 細胞性粘菌の脱分化に伴う細胞内pH変化の計測 (2020)
  • Author(s): 臼井友美，森本雄祐
  • Organizer: 日本顕微学会 第76回学術講演会
• [Presentation] Measurement of cytosolic pH changes during dedifferentiation of Dictyostelium cells (2020)
  • Author(s): Tomomi Usui, Yusuke V. Morimoto
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] 細胞性粘菌の多細胞システムにおける機械刺激応答機構 (2020)
  • Author(s): 森本雄祐, 橋村秀典, 平山悠成, 上田昌宏
  • Organizer: 第62 回 日本顕微鏡学会九州支部学術講演会
• [Presentation] 細胞性粘菌の多細胞体における機械刺激応答機構 (2020)
  • Author(s): 森本雄祐, 橋村秀典, 平山悠成, 上田昌宏
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] 細胞性粘菌の多細胞体における機械刺激受容メカニズム (2020)
  • Author(s): 橋村秀典, 平山悠成, 上田昌宏, 森本雄祐
  • Organizer: 2020年 生体運動研究合同班会議
• [Presentation] 生命機能を理解するための高感度細胞質pHイメージング (2019)
  • Author(s): 森本雄祐
  • Organizer: 第43回 蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Invited
• [Presentation] Detection of cell differentiation states without known signals in Dictyostelium (2019)
  • Author(s): Yusuke V. Morimoto, Takuro Kawada, Masahiro Ueda
  • Organizer: 第57回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] 細胞性粘菌における自家蛍光を用いた細胞分化状態の検出 (2019)
  • Author(s): 森本雄祐
  • Organizer: 第9回 日本細胞性粘菌学会例会
• [Presentation] 細胞性粘菌のcAMPシグナルリレーを調節する膜電位変化 (2019)
  • Author(s): 森本雄祐, 上田昌宏
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] 細胞細胞性粘菌における機械刺激応答メカニズムの解明 (2019)
  • Author(s): 平山悠成, 橋村秀典, 上田昌宏, 森本雄祐
  • Organizer: 第61回日本顕微鏡学会九州支部学術講演会
• [Presentation] 細胞性粘菌の脱分化過程における細胞質pH変化の計測 (2019)
  • Author(s): 臼井友美, 森本 雄祐
  • Organizer: 第61回日本顕微鏡学会九州支部学術講演会
• [Presentation] 細胞性粘菌の分化に伴った細胞内環境遷移の可視化 (2019)
  • Author(s): 森本雄祐, 川田拓朗, 上田昌宏
  • Organizer: 第61回日本顕微鏡学会九州支部学術講演会
• [Presentation] 細胞細胞性粘菌における機械刺激応答メカニズムの解明 (2019)
  • Author(s): 平山悠成, 橋村秀典, 上田昌宏, 森本雄祐
  • Organizer: 日本生体エネルギー研究会第45回討論会
• [Presentation] 細胞性粘菌の脱分化過程における細胞質pH変化の計測 (2019)
  • Author(s): 臼井友美, 森本 雄祐
  • Organizer: 日本生体エネルギー研究会第45回討論会
• [Presentation] 細胞性粘菌の多細胞動態にcAMP振動は必須ではない (2019)
  • Author(s): 橋村秀典、森本雄祐、安井真人、上田昌宏
  • Organizer: 2019年生体運動研究合同班会議
• [Presentation] 細胞性粘菌のcAMPシグナルリレーを調節する膜電位変化 (2018)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 日本顕微鏡学会第74回学術講演会
• [Presentation] 細胞性粘菌の細胞分化に伴う細胞質pH変化 (2018)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 第５６回日本生物物理学会年会
• [Presentation] 細胞性粘菌における単細胞から多細胞へのシグナル伝達様式の変遷 (2018)
  • Author(s): 橋村秀典、森本雄祐、安井真人、上田昌宏
  • Organizer: 日本生体エネルギー研究会第４４回討論会