Structural and functional analyses of the respiratory terminal enzymes for elucidation of the proton pump mechanism
| Project/Area Number |
18K06162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | University of Hyogo |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生体エネルギー / 生物物理 / 呼吸鎖 / 膜蛋白質 / 酵素 / X線結晶解析 / 電顕単粒子解析 / ナノバイオ / 蛋白質 |
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| Outline of Final Research Achievements |
Respiratory chains in mitochondrial and bacterial membrane transfer hydrogen ions (protons) by using energy released from the redox reaction (electron transfer), and efficiently generates energy available for the life. To understand the energy transduction mechanism of the respiratory terminal enzymes, I studied molecular structure and function of mitochondrial cytochrome c oxidase and bacterial nitric oxide reductases. The resolution of structural analysis was improved. The results showed structural changes of the enzymes depending on the reaction with the substrates and inhibitors, and indicated the regulation mechanism of enzymatic activity depending on the monomerization/dimerization of enzyme.
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| Academic Significance and Societal Importance of the Research Achievements |
呼吸鎖酵素は生体膜を介したプロトン移動による効率的なエネルギー変換を行う。ミトコンドリア呼吸鎖の代謝異常は活性酸素産生などに関係する。病原菌の呼吸鎖酵素は宿主免疫系から菌体を守る働きを担う。本研究では呼吸鎖酵素のエネルギー変換機構に関して分子レベルでの知見が得られたとともに、これらの知見は高効率なエネルギー変換素子の構築、代謝異常のメカニズム解明、病原菌に対する薬剤の創出につながることが期待される。
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Monomeric structure of an active form of bovine cytochrome c oxidase2019
Author(s)
Shinzawa-Itoh Kyoko、Sugimura Takashi、Misaki Tomonori、Tadehara Yoshiki、Yamamoto Shogo、Hanada Makoto、Yano Naomine、Nakagawa Tetsuya、Uene Shigefumi、Yamada Takara、Aoyama Hiroshi、Yamashita Eiki、Tsukihara Tomitake、Yoshikawa Shinya、Muramoto Kazumasa
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 116
Issue: 40
Pages: 19945-19951
DOI
Related Report
Peer Reviewed / Open Access
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