Exploring the mechanism behind environmental adaptation of circadian clock in cyanobacteria

• Project/Area Number: 18K06171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: Institute for Molecular Science
• Principal Investigator: Mukaiyama Atsushi 分子科学研究所, 協奏分子システム研究センター, 助教 (80647446)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生物時計 / シアノバクテリア / KaiC / ATPase / 概日時計 / 時計タンパク質 / 進化・多様性 / 分子進化

Outline of Final Research Achievements

This project aims to clarify the mechanism underlying the evolutionary diversity of cyanobacterial circadian clock.
The cyanobacterial circadian clock is composed of the three kinds of proteins (KaiA, KaiB and KaiC), and the periodicity is encoded in KaiC solely. Biochemical analysis of diverse KaiC homologues suggests that KaiC has acquired its clock function through a stepwise evolutionary process.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果から、現在のシアノバクテリア生物時計が段階的な進化を経て形作られたことが示唆された。今後、その詳細を解析することで生物時計の起源を知る手がかりが得られると予想される。

Research Products

• [Journal Article] Development and optimization of expression, purification and ATPase assay of KaiC for medium throughput screening of circadian clock mutants in cyanobacteria (2019)
  • Author(s): Ouyang D, Furuike Y, Mukaiyama A, Ito-Miwa K, Kondo T and Akiyama S
  • Journal Title: Int. J. Mol. Sci. Volume: 20 Issue: 11 Pages: 2789-2789
  • DOI: 10.3390/ijms20112789
  • Peer Reviewed / Open Access
• [Journal Article] KaiC from a cyanobacterium Gloeocapsa sp. PCC 7428 retains functional and structural properties required as the core of circadian clock system. (2019)
  • Author(s): Mukaiyama A, Ouyang D, Furuike Y, Akiyama S
  • Journal Title: International Journal of Biological Macromolecules Volume: 131 Pages: 67-73
  • DOI: 10.1016/j.ijbiomac.2019.03.051
  • Peer Reviewed / Open Access
• [Journal Article] Conformational rearrangements of the C1 ring in KaiC measure the timing of assembly with KaiB (2018)
  • Author(s): Mukaiyama A, Furuike Y, Abe J, Yamashita E, Kondo T, Akiyama S
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 8803-8803
  • DOI: 10.1038/s41598-018-27131-8
  • Peer Reviewed / Open Access
• [Presentation] シアノバクテリア概日時計における進化的多様性の分子メカニズム (2021)
  • Author(s): 向山 厚、古池 美彦、秋山 修志
  • Organizer: 令和2年度生物物理中部支部講演会
• [Presentation] Disassembly kinetics of the KaiBC complex in the cyanobacterial clock system (2021)
  • Author(s): D. Simon, A. Mukaiyama, Y. Furuike and S. Akiyama
  • Organizer: 令和2年度生物物理中部支部講演会
• [Presentation] 多種KaiCの機能解析によるシアノバクテリア生物時計の進化的多様性の考察 (2019)
  • Author(s): 向山 厚、欧陽 東彦、古池 美彦、秋山 修志
  • Organizer: 第19回日本蛋白質科学会年会
• [Presentation] 機能解析から探るKaiタンパク質時計システムの進化的多様性 (2019)
  • Author(s): 向山 厚
  • Organizer: Cyanoclock2.0
• [Presentation] Seeking for evolutionary diversity of Kai-protein clock system in cyanobacteria (2019)
  • Author(s): Mukaiyama A, Ouyang D, Furuike Y and Akiyama S
  • Organizer: XVI European biological rhythm society congress
  • Int'l Joint Research
• [Presentation] Evolutionary diversity of Kai-protein clock in cyanobacteria (2019)
  • Author(s): Mukaiyama A, Ouyang D, Furuike Y and Akiyama S
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] 他種KaiCの機能解析から探るシアノバクテリア生物時計システムの進化的多様性 (2019)
  • Author(s): 向山 厚、秋山 修志
  • Organizer: 第26回日本時間生物学会学術大会
  • Invited
• [Presentation] シアノバクテリアGloeocapsa sp. PCC 7428株由来KaiCの機能解析 (2019)
  • Author(s): 向山 厚、欧陽 東彦、古池 美彦、秋山 修志
  • Organizer: 平成30年度日本生物物理学会中部支部講演会
• [Presentation] Conformational rearrangements of the C1 ring in KaiC measure the timing of assembly with KaiB via a conformational selection mechanism (2018)
  • Author(s): Atsushi Mukaiyama, Yoshihiko Furuike, Eiki Yamashita, Takao Kondo and Shuji Akiyama
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] シアノバクテリア生物時計の分子起源とその進化 (2018)
  • Author(s): 向山 厚、古池 美彦、秋山 修志
  • Organizer: 第25回日本時間生物学会学術大会
  • Invited
• [Book] Biological Rhythms (2019)
  • Author(s): Mukaiyama A, Furuike Y and AkiyamaS (分担執筆）
  • Total Pages: 164
  • Publisher: 北海道大学出版会
  • ISBN: 9784832903746