Direct visualization of functional basis in voltage-dependent ion-channels
Project/Area Number |
18K06172
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 43040:Biophysics-related
|
Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Shigematsu Hideki 国立研究開発法人理化学研究所, 放射光科学研究センター, 研究員 (00415928)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | クライオ電子顕微鏡 / 構造生物学 / イオンチャネル / 電位依存性 / 膜電位 / 可視化 / 構造・機能相関 |
Outline of Final Research Achievements |
Conformational change in ion-pore has been revealed directly by using single particle electron cryo-microscopy in this study. The specimen used for image analysis had been utilized in functional analysis by using florescent spectroscopy and it revealed that it can maintain membrane potential in a few minutes. The method here can be applied to any other membrane proteins to see its function on close to physiological environment, liposome.
|
Academic Significance and Societal Importance of the Research Achievements |
リポソーム上に提示された膜タンパク質の機能構造をクライオ電子顕微鏡で明らかにすることは、今後の医薬品開発への応用や、実際に細胞が機能する現場を捉えるという基礎学問的な意義も重要である。
|
Report
(4 results)
Research Products
(18 results)
-
-
-
-
[Journal Article] Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein.2021
Author(s)
Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T.
-
Journal Title
Structure
Volume: 29
Issue: 3
Pages: 252-260
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Structural analysis reveals TLR7 dynamics underlying antagonism2020
Author(s)
Tojo Shingo、Zhang Zhikuan、Matsui Hiroyuki、Tahara Masahiro、Ikeguchi Mitsunori、Kochi Mami、Kamada Mami、Shigematsu Hideki、Tsutsumi Akihisa、Adachi Naruhiko、Shibata Takuma、Yamamoto Masaki、Kikkawa Masahide、Senda Toshiya、Isobe Yoshiaki、Ohto Umeharu、Shimizu Toshiyuki
-
Journal Title
Nature Communications
Volume: 11
Issue: 1
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] HCV IRES captures an actively translating 80S ribosome2019
Author(s)
Yokoyama Takeshi、Machida Kodai、Iwasaki Wakana、Shigeta Tomoaki、Nishimoto Madoka、Takahashi Mari、Sakamoto Ayako、Yonemochi Mayumi、Harada Yoshie、Shigematsu Hideki、Shirouzu Mikako、Tadakuma Hisashi、Imataka Hiroaki、Ito Takuhiro
-
Journal Title
Molecular Cell
Volume: 印刷中
Issue: 6
Pages: 1205-1214
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-